“肺主呼吸”对“通调水道”影响的实验观察

The Experimental Observation of effect of “Lung Governing Respiration” on “Regulating Waterway”

目的:观察呼吸功能改变与尿量变化之间的相关性,探讨观察"肺主呼吸"对"通调水道"的影响。方法:以尿液量变化为主要指标,观察哮喘模型小鼠呼吸功能改变、慢性阻塞性肺疾病(Chronic Obstructive Pulmonary Diseases,COPD)模型呼吸大鼠功能改变及正压扩肺家兔肺通气活动改变对"通调水道"的影响。测量各动物特定时段的产尿量,观察模型组动物尿量是否较对照组有所改变。结果:3个动物实验模型组动物尿量均较对照组减少。1)哮喘模型小鼠5 h总尿量较对照组减少(P<0.05);2)COPD模型大鼠在6 h,12 h,18 h和24 h各时段的总尿量均较对照组减少(P<0.05);3)正压扩肺家兔尿量较自身平静呼吸时10 min内尿滴数减少(P<0.05),且正压扩肺家兔正压扩肺干预后10 min内尿滴数较对照组减少(P<0.05)。结论:获得了"肺主呼吸"对"通调水道"的影响实验依据,为进一步深入探讨"肺主通调水道"相关分子信号的调控途径,揭示其现代生物学机制奠定了基础。

Abstract Objective：To observe the correlation between respiratory function and urine volume change and to explore the influence of ＂lung governing respiration＂ on ＂regulating waterway＂. Methods：The effects of changes in urine volume, as the main index, respiratory function changes of asthma mice model and chronic obstructive lung disease （COPD） model and positive pressure ex- panding lung in rabbit pulmoual ventilatory activity changes on ＂regulating waterway＂ were observed. Urine volume of each ani- mal was measured set for a specific time period and urine volume of model groups was compared with that of the control group. Re- sults：The urine volumes of three experimental animal models were lower than those in the control group. 1 ） Five-hour total urine volume of asthma mice model of was less than that of the control group （P 〈 0. 05）. 2） Total volume urine of COPD rat model at 6 h, 12 h, 18 h and 24 h separately were higher than those of the control group （P 〈 0. 05 ）. 3 ） Urine drop number of positive pressure lung-expanding rabbits reduced compared to that during their 10 min normal breathing （ P 〈 0. 05 ） and the one after 10 min expending was also decreased compared with that of the control group （P 〈 0. 05 ）. Conclusion：The experimental basis of the eftct of ＂lung governing respiration＂ on ＂regulating waterway＂ was obtained to further explore the related molecular signaling pathways, providing foundation for modern biological mechanism.