黑粒子胶囊对小鼠肝癌H22移植性实体瘤生长的影响

Effects and mechanism black granule capsules on growth of transplanted H22 mouse hepatocarcinoma cells

目的：研究黑粒子胶囊对小鼠肝癌H22移植性实体瘤的生长及细胞增殖周期和凋亡的影响。方法将肝癌细胞H22细胞接种于小鼠皮下，接种后24 h将已接种的癌瘤细胞小鼠按体重随机分为空白对照组，阳性对照组，黑粒子胶囊低、中、高剂量组，每组12只。阳性对照组腹腔注射环磷酰胺溶液30 mg/kg，黑粒子胶囊低、中、高剂量组分别灌胃黑粒子胶囊混悬液400、800、1600 mg/kg，空白对照组灌胃等体积生理盐水，给药1次/d，连续给药7 d，末次给药后24 h颈椎脱臼处死小鼠，计算抑瘤率，并使用流式细胞仪检测H22实体瘤的细胞增殖周期和凋亡情况。结果黑粒子胶囊高剂量组对实体瘤生长有明显抑制作用，雄性组抑瘤率为38.78%，雌性组抑瘤率为43.57%。与空白对照组比较，黑粒子胶囊低、中、高剂量组肿瘤细胞G0～G1期[（58.06±9.65）%、（55.10±5.89）%、（61.36±15.95）%比（74.47±2.63）%]缩短、S 期[（33.96±11.90）%、（32.67±4.04）%、（33.89±9.82）%比（14.37±4.92）%]延长，细胞凋亡率[（31.12±1.85）%、（31.89±2.16）%、（40.64±0.55）%比（21.75±2.64）%]增高。结论黑粒子胶囊可抑制小鼠肝癌H22移植性实体瘤的生长，其抑瘤作用机制可能与影响细胞增殖周期特别是与诱导细胞凋亡有关。

Objective To study the effect of black granule capsules（BGCs） on growth of transplanted mouse hepatocarcinoma cells, cell proliferation cycle and apoptosis.Methods KM mouse were subcutaneously inoculated with Hepatocarcinoma cells （H22） and randomly divided into the model control group, the positive control group, the low, medium and high does of BGC group after 24h. The positive control group received intraperitoneal injection with 30 mg/kg cyclophosphamide. Mice of BGC groups were intragastricaly with different dosage of BGC （400, 800, 1 600 mg/kg）. The model control group received intragastricaly with normal saline. The drugs were administrated once a day for seven days. The tumor inhibition rate was calculated at 24 h after the last administration. Flow cytometry was used to detect changes of cell cycle and apoptosis in harvested H22 tumor cells.Results The group of high does showed significant inhibitory effect on the growth of transplanted H22 tumor cells withthe inhibitory rate 38.78% （male） and 43.57% （female）. Compared with model control group, groups of different dosages decreased time of G0-G1 phase （58.06% ± 9.65%, 55.10% ± 5.89%, 61.36% ± 15.95%vs. 74.47% ± 2.63%）, increased tiem of Sphase （33.96% ± 11.90%, 32.67% ± 4.04%, 33.89% ± 9.82%vs. 14.37% ± 4.92%）, and increased the apoptosis rate （31.12% ± 1.85%, 31.89% ± 2.16%, 40.64% ± 0.55%vs.21.75% ± 2.64%）.Conclusion BGC has antitumor effect on mouse hepatocarcinoma H22 tumor cells, and its mechanism was to regulate cell proliferation cycle and induce the apoptosis.