木犀草素对急性汞中毒小鼠血液生化指标、肝脏及肾脏的影响

Effect of Luteolin on Blood Indexes,Liver and Kidney in Mice with Acute Mercury Poisoning

试验旨在研究小鼠急性汞中毒后血液、肝脏及肾脏的中毒表现及木犀草素对急性汞中毒小鼠血液指标、肝脏及肾脏的影响。将28只小鼠随机分成4组,分别为对照组(腹腔注射0.9%生理盐水)、木犀草素组(灌胃100mg/kg木犀草素)、氯化汞组(腹腔注射4mg/kg氯化汞)和氯化汞+木犀草素组(腹腔注射4mg/kg氯化汞,灌胃100mg/kg木犀草素)。检测血清谷丙转氨酶(ALT)和谷草转氨酶(AST)活性,肌酐(CREA)和尿素氮(BUN)水平,血液白细胞(WBC)、红细胞(RBC)和血红蛋白(HGB)水平及肝脏组织谷胱甘肽(GSH)和丙二醛(MDA)水平,并观察肝脏、肾脏组织形态学改变。结果显示,与对照组相比,氯化汞组的血清ALT、AST活性极显著升高(P<0.01),血清CREA、BUN,血液WBC及肝脏组织MDA含量均显著增加(P<0.05),血液RBC、HGB及肝脏组织GSH水平均显著降低(P<0.05),肝脏、肾脏病理变化明显。与氯化汞组相比,氯化汞+木犀草素组的血清ALT、AST活性,CREA和BUN,血液WBC及肝脏组织MDA含量均显著降低(P<0.05);血液RBC、HGB及肝脏组织GSH含量均显著升高(P<0.05),肝脏、肾脏病理变化减轻。小鼠急性汞中毒后,其中毒表现为炎症和贫血的发生及肝脏、肾脏不同程度的损伤,木犀草素可减弱急性汞中毒小鼠血液、肝脏和肾脏的毒性作用。

To study the effect of luteolin on blood indexes,liver and kidney in mice with acute mercury poisoning,28 mice were randomly divided into four groups：Control group（intraperitoneal injection 0.9% saline）,luteolin group（lavage 100 mg/kg luteolin）,mercuric chloride group（intraperitoneal injection 4mg/kg mercury chloride）and mercury chloride＋luteolin group（intraperitoneal injection 4mg/kg mercuric chloride,lavage 100mg/kg luteolin）.The activities of ALT in serum,AST,CREA and BUN contents,blood WBC,RBC,HGB content and GSH and MDA contents of liver tissue were detected.Morphological changes of liver and kidney tissues were observed.The results showed that compared with the control group,the activities of ALT and AST of mercuric chloride group were extremely significantly increased（P〈0.01）,serum CREA,BUN,blood WBC and liver tissue MDA contents were significantly increased（P〈0.05）while blood RBC,HGB and liver tissue GSH contents were significantly decreased（P〈0.05）.Liver,kidney pathological changes were obviously.Compared with mercuric chloride group,the activities of ALT,AST in serum,CREA,BUN,blood WBC and liver tissue MDA contents of mercuric chlo-ride＋luteolin group were significantly decreased（P〈0.05）,while blood RBC,HGB and liver tissue GSH contents were significantly elevated（P〈0.05）.Liver,kidney pathological changes were attenuated obviously.The poisoning were characterized by inflammation and the occurrence of anemia when acute mercury poisoning occurred,liver and kidney showed different degrees of injury in mice.Luteolin could reduce the toxic effects of acute mercury poisoning on blood,liver and kidney.