红树植物老鼠簕内生真菌Aspergillus flavipes AIL8化学成分的研究

Chemical Constituents of an Endophytic Fungus Aspergillus flavipes AIL8 obtained from Mangrove Acanthus ilicifolius

利用硅胶柱层析、Sephadex LH-20凝胶柱层析及半制备高效液相色谱等分离手段对红树林老鼠簕内生真菌Aspergillus flavipes AIL8的提取物进行分离纯化,并利用各种波谱方法,结合文献对照,确定化合物结构。从提取物中分离得到6个化合物,经鉴定为4-羟基-3-甲氧基-5-[(2E,6E)-3,7,11-三甲基十二烷-2,6,10-三烯-1-基]苯甲酸(1)、芳香类内酯化合物(2)、一癸酸甘油酯(3)、菜子甾醇(4)、7,22(E)-麦角二烯-3β,5α,6β-三醇(5)和麦角甾-6,22-二烯-5α,8α-环二氧-3β-醇(6)。6个化合物均为首次从红树林老鼠簕内生真菌A.flavipes AIL8中分离得到,其中化合物1为新天然产物。

In this study,the chemical constituents of an endophytic fungus AspergiUus flavipes AIL8 obtained from man- grove Acanthus ilicifolius were isolated and purified by column cln＇omatography on silica gel, Sephadex LH-20 and semi- preparative HPLC. The structures of the isolated compounds were determined on the basis of spectroscopic analysis and comparison with literature data. Six compounds were identified as 4-hydroxy-3-methoxy-5-（ （2E, 6E）-3,7,11-trimeth- yldodeca -2,6,10-trien-l-yl） benzoic acid （ 1 ）, aromatic lactone derivative （ 2 ）, 2,3-dihydroxypropyl decanoate （3）, brassicasterol （4）, 7,22 （ E ） -ergostadiene-3β, 5 α, 6β-triol （ 5 ） and ergosterol-6,22-diene-Sa, 8a-epidioxy-3β-ol （ 6 ）. All the 6 compounds were isolated from this strain for the first time,in which compound 1 was a new natural product.