HPLC法同时测定苍耳类药材中酚酸及蒽醌类成分的含量

Simultaneous Determination of Phenolic Acids and Anthraquinones in Xanthii Herba and Xanthii Fructus by High Performance Liquid Chromatography

建立HPLC同时测定苍耳子、苍耳草药材中绿原酸、新绿原酸、原儿茶醛、原儿茶酸、隐绿原酸、咖啡酸、1,3-二咖啡酰奎宁酸、阿魏酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸、芦荟大黄素、大黄素及大黄酚含量的方法。采用Agilent ZORBAX SB-C18色谱柱（250×4.6 mm,5μm）,以甲醇（A）-0.1%甲酸水溶液（B）为流动相,梯度洗脱,流速1.0 m L/min,柱温35℃,检测波长254 nm,进样量10μL。14种成分的浓度与峰面积的线性关系良好（r〉0.9931）;加样回收率为97.18%~101.09%。苍耳类药材中酚酸和蒽醌类14种成分的含量存在差异,其中绿元素、原儿茶酸含量较高,芦荟大黄素、大黄素、大黄酚含量较低。该方法简单、准确,重现性较好,可用于苍耳类药材内在质量的评价和控制。

To establish a high performance liquid chromatography （HPLC） method for the simultaneous determination of chlorogenic acid, neochlorogenic acid, nprotocatechualdehyde, nprotocatechuienacid, cryptochlorogenic acid, caffeic acid, 1,3-dicaffeoylquinic acid,ferulic acid,3,4-dicaffeoylquinic acid ,3,5-dicaffeoylquinic acid,4,5-dicaffeoylquinic acid, al- oe emodin,emodin and ehrysophanol in Xanthii Herba and Xanthii Fruetus. The assay was conducted on an Agilent ZORBAX SB-C,8 column （250 μ4.6 mm,5 μm） with methanol-0.1% formic acid in water as mobile phases. The flow rate was 1.0 mL/min, the detection wavelength was 254 nm and the column oven temperature was 35 ℃. The injection volume was 10 μL. The calibration curves of the 14 constituents showed good linearity （ r 〉 0.9931 ） within the ranges of the tested concentrations. The average recoveries （n = 6） of 14 constituents were 97. 18%-101.09%. The results showed that the contents of these 14 constituents varied in Xanthii Herba and Xanthii Fructus. The contents of chlorogen- ic acid and protocatechuic acid were the highest,while the contents of aloe emodin, cmodin and chrysophanol were low- est. The developed method was simple, accurate and reproducible. It can be used for the quality evaluation and control of Xanthii Herba and Xanthii Fructus.