摘要
Brucellosis is a worldwide zoonosis caused by Brucella, which poses great threat to human health. Totally 234 milk samples from a scale dairy farm were amplified by nested PCR, and the pathogens were further separated. The results showed that a DNA band of 419 bp was amplified from 18 out ot＂234 milk samples. Among 18 positive milk samples, nine samples amplified the DNA band of 535 bp, which were B. su/s ; one sample amplified the DNA bands of 535 bp and 285 bp, which was the mixture of B. su/s and B. bov/s. The results laid a foundation for prevention and control of brucellosis.
Brucellosis is a worldwide zoonosis caused by Brucella, which poses great threat to human health. Totally 234 milk samples from a scale dairy farm were amplified by nested PCR, and the pathogens were further separated. The results showed that a DNA band of 419 bp was amplified from 18 out ot＂234 milk samples. Among 18 positive milk samples, nine samples amplified the DNA band of 535 bp, which were B. su/s ; one sample amplified the DNA bands of 535 bp and 285 bp, which was the mixture of B. su/s and B. bov/s. The results laid a foundation for prevention and control of brucellosis.
