猕猴桃褐腐病生防菌株GMH31发酵条件的优化

Fermentation Optimization of Pestalotiopsis uvicola GMH31 against Botryosphaeria dothidea on Kiwifruit

利用从药用植物牡蒿(Artemisia japonica)中分离筛选出的1株猕猴桃褐腐病生防菌株GMH31,采用含毒介质平板法进行抑菌试验,通过单因子试验和正交试验,对该菌株的最适发酵培养基成分(包括碳源、氮源、无机盐离子、微量元素)及发酵条件(包括发酵时间、温度、pH、转数、装液量、接种量)进行了优化。结果显示:尤韦可拟盘多毛孢菌株GMH31培养基组分最佳配比为麦芽糖35 g/L、氯化铵12 g/L、K_2HPO_40.1 g/L、甘氨酸0.015 g/L,最佳发酵条件为培养时间14 d、培养温度26℃、初始pH 6.0、摇床转速120 r/min、250 m L装液量100 m L、接菌量为装液量的6%。在最佳发酵培养基和培养条件下,GMH31菌株发酵滤液对猕猴桃褐腐病菌的抑制率提高了16.45%。

Strain GMH31 was Pestalotiopsis uvicola isolated from Artemisia japonica,and it could control efficiently Botryosphaeria dothidea on kiwifruit. Antifungal active test was studied by poison medium technique. Single factor experiment and orthogonal experiment were adopted to optimize liquid fermentation media（ carbon source,nitrogen source,inorganic ions and trace elements） and conditions（ fermentation time,culture temperature,p H,rotating speed,medium capacity and inoculums amount）. The results showed that the optimal compositions of cultural medium were as follows： maltose 35 g / L,NH4Cl 12 g / L,K2HPO4 0. 1 g / L and glycine 0. 015 g / L. The optimal fermentation conditions were as follows： fermentation time 14 d,cultured temperature 26 ℃,initial pH 6. 0,rotating speed 120 r / min,medium capacity 100 / 250 mL,and inoculation rate 6%. Inhibitory rate of strain GMH31 fermentation filtrate against Botryosphaeria dothidea on kiwifruit increased by 16. 45% under the optimal fermentation medium and culture conditions.