聚三亚甲基碳酸酯的生物相容性研究

Investigation on the Biocompatibility of Poly(trimethylene carbonate)

以实验室自制的聚三亚甲基碳酸酯(PTMC)为研究对象,通过测定聚三亚甲基碳酸酯在体外酶解过程中降解液pH变化,考察其在降解过程中是否产生酸性降解产物;通过MTS法考察聚三亚甲基碳酸酯的体外细胞毒性;通过HE染色的方法考察聚三亚甲基碳酸酯在大鼠体内埋植部位的皮肤刺激性,进而考察聚三亚甲基碳酸酯的生物相容性。结果表明:PTMC在降解过程中不产生酸性降解产物,可避免埋植部位无菌炎症的产生。同时不同分子量的PTMC可以存在于皮下组织而不会造成伤害,因此聚三亚甲基碳酸酯具有良好的生物相容性,可安全植入体内。

The PTMC rods were directly synthesized via a bulk ring-opening polymerization reaction of TMC in the presence of Sn（Oct）e as the catalys. The pH of the media before and after degradation was monitored with a combination microelectrode （InLabMieroTM, Toledo-Mettler） and a digital pH meter （FiveEasyTM, Toledo-Mettler）. The potential cytotoxicity of the PTMC rods was evaluated using an MTS assay. For one polymer at each study time point, six specimens were implanted bilaterally into the subcutaneous tissue of the back of one rat. After implantation, the Histology of the polymers was evaluated by H＆E. The results showed that PTMC can be degraded without the formation of the acidic degradation products, and all the PTMC rods can thus be considered non-cytotoxic with no adverse effects on cell viability. In addition, the PTMC rods are able to coexist with subcutaneous tissues without causing harm, regardless of their initial molecular weight. Thus, the PTMC rods are considered to have good biocompatible and safe for in vivo implantation.