木丹颗粒对糖尿病大鼠背根神经节中活化的Caspase-3、Bcl-2、Bax蛋白表达的影响

The Effects of Mudan Granula(木丹颗粒) on Protein Expression of Caspase-3, Bax and Bcl-2 of the Dorsal Root Ganglion Neuron in Diabetic Rats

目的:探讨木丹颗粒对链脲佐菌素(STZ)诱导的糖尿病大鼠背根神经节中活化的Caspase-3、Bcl-2、Bax蛋白表达的影响。方法:将60只大鼠分为正常组、模型组、甲钴胺片组和木丹颗粒组,采用STZ诱导复制糖尿病模型,正常组和模型组灌胃生理盐水,甲钴胺片组和木丹颗粒组分别灌胃甲钴胺片和木丹颗粒,于干预4周后处死大鼠,取背根神经节。对比各组大鼠背根神经节中活化的Caspase-3、Bcl-2、Bax蛋白表达情况。结果:模型组活化Caspase-3、Bax蛋白表达高于正常组(P<0.01),Bcl-2蛋白表达明显低于正常组(P<0.01);木丹颗粒组与甲钴胺片组大鼠背根神经节中活化的Caspase-3、Bax蛋白表达均明显低于模型组(P<0.01),Bcl-2蛋白表达均显著高于模型组(P<0.01);木丹颗粒组与甲钴胺片组比较,差异无统计学意义(P>0.05)。结论:木丹颗粒能抑制糖尿病大鼠背根神经节中Caspase-3的活化,下调Bax的表达,上调背根神经节中Bcl-2的表达,可抑制Caspase-3诱导的细胞凋亡启动程序。

Objective： To explore the effects of Mudan Granula on protein expression of Caspase-3, Bax and Bcl-2of the dorsal root ganglion neuron in STZ-induced diabetic rats. Methods： The 60 rats were divided into normal control group, model group, mecobalamin group and Mudan Granula group. The diabetic rats were in- duced with STZ. The rats were given normal saline in normal control group and model group, and Mecobalamin piece sets and Mudan Granula respectively in Mecobalamin group and Mudan Granula group. The protein ex- pression of Caspase-3, Bax and Bcl-2 of the dorsal root ganglion neuron were contrasted in 4 weeks. Results： The model group showed protein higher expression of Caspase-3 and Bax, as well as lower Bcl-2 than the nor- mal control group （P〈0.01）. The mecobalamin group and Mudan Granula group showed lower protein expression of Caspase-3 and Bax, as well as higher Bcl-2 than the model group （P〈0.01）; there was no statistical differ- ence between the mecobalamin and Mudan Granula groups （P〉0.05）. Conclusions： Mudan Granula can inhibit ac- tivation of Caspase-3, down-regulate the expression of Baxand, up-regulate the expression of Bcl-2, and inhibit the apoptosis induced by Caspase-3.