摘要
目的：建立复方酸枣仁膏质量标准。方法：采用TLC法鉴别川芎、甘草;采用HPLC法测定本制剂中阿魏酸的含量。色谱条件：流动相为甲醇-水-冰醋酸（22∶78∶1）,检测波长为323 nm,流速为0.6 m L/min;柱温为室温。结果：采用的薄层色谱斑点清晰,分离度较好,阴性无干扰。HPLC法检测阿魏酸的线性范围为0.02650~0.47670μg,平均回收率为96.81%。结论：本实验方法简单,重复性好,可进行定性、定量检测,用于复方酸枣仁膏的质量控制。
Objectives： To establish the quality standards for the paste of compounded spine date seed. Methods： Gancao （Licorice） and Chuanxion （Ligusticum Chuanxiong） were identified by the thin-layer chromatog- raphy （TLC）. The content of the ferulic acid in the preparation was formulated by the high performance liquid chromatography （HPLC）. Mobile Phase was Methanol-Water-Acetic Acid （22：78：1）; Detected Wavelength was 323 nm; Current Velocity was 0.6 mL/min; column temperature was room temperature. Results： The clear dappled spot. Good separation and no negative interference in the miscellaneous TLC. The linear range is 0.02650- 0.47670 μg for the ferulic acid by the HPLC. The RSD is 1.29%. The average recovery rate is 96.81%. Con- clusions： The establishment of the quality standards. The methods could be used for quality controlling of the paste of compounded spine date seed.
出处
《中医药导报》
2016年第13期101-103,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
关键词
复方酸枣仁膏
阿魏酸
薄层色谱
高效液相色谱
The paste of the compounded spine date seed
ferulic acid
thin-layer chromatography （TLC）
high performance liquid chromatography （HPLC）
