肝细胞生长因子对大鼠心肌细胞动作电位的作用

Effect of hepatocyte growth factor on the action potential duration of ventricular myocytes in rat

目的研究肝细胞生长因子(HGF)对大鼠心肌细胞动作电位时程(APD)的影响。方法酶解法分离大鼠心肌细胞,随机分成对照组(HGF,0)和3个质量浓度实验组(HGF,5,10,50μg·m L^(-1)),以异丙肾上腺素(ISO)作为刺激因素,用膜片钳技术,分别记录各组心肌细胞在HGF作用下、当动作电位复极50%,90%所需时间(APD_(50)、APD_(90))。结果 ISO可明显延长对照组细胞的APD。与对照组相比,小、中、大3个质量浓度实验组细胞的APD_(50)的延长率分别下降1.2%,42.3%,49.2%,同时APD_(90)的延长率分别下降19%,41.1%,48.8%,HGF对抗ISO的作用成浓度依赖性增强趋势;而在不给予ISO刺激的情况下,与对照组相比,3个质量浓度实验组心肌细胞的APD_(90)分别缩短6.8%,13.3%,28.2%,HGF的作用同样呈浓度依赖性增强趋势。结论 HGF可缩短APD,促进细胞膜复极,具有对抗心肌肥大时的电重构潜力,有助于心肌肥厚的治疗。

Objective To study the effect of hepatocyte growth factor （HGF） on the action potential duration （APD） of ventricular myocytes in rat. Methods Myocytes were isolated by collagenase and were randomly divided into control group （ HGF, 0 ） and three concentration test groups （5, 10, 50 μg . mL-1HGF）. With isoprenaline （ISO） as stimulating factor, patch clamp technique was used to record the APDs0 and APDgo in ceils incubated by HGF. Results APD in control group were significantly prolonged by ISO. Compared with the control group, the prolongation of APD50 in small, middle and large test groups decreased 1.2%, 42.3% and 49.2%, while the one of APDgo decreased 19%, 41.1% and 48.8%, respectively as the increasing dose of HGF. The effect of HGF against ISO tends to be concentration dependent. Without ISO, APD90 in three test groups were shortened 6. 8%, 13.3% and 28.2% respectively by HGF compared with control group, as the similar trend. Conclusion HGF could shorten APD and promote repolarization, which might have the potential to resist the electrical remodeling when cardiac hypertrophy that was helpful to the treatment of cardiac hypertrophy.