LIM结构域结合蛋白1在脑胶质瘤患者中的表达研究

The Study of LIM-domain-binding 1 Expression in Brain of Patients with Gliomas

目的观察LIM结构域结合蛋白1(LIM-domain-binding 1,Ldb1)在脑胶质瘤患者中的表达。方法采用Real-time PCR方法检测46例脑胶质瘤手术患者脑组织、18例正常对照和16例良性脑肿瘤对照脑组织标本中Ldb1 mRNA的表达水平;采用免疫组化En Vision二步法观察Ldb1在高级别胶质瘤中的蛋白表达定位。结果脑胶质瘤患者脑组织Ldb1的mRNA表达高于正常对照组和良性脑肿瘤对照组。Ldb1在不同分级的胶质瘤中表达并不相同,在III、IV级中的相对表达量明显高于Ⅰ、Ⅱ级(P<0.001)。在高级别胶质瘤中,Ldb1蛋白主要表达在肿瘤细胞核和胞浆内。结论脑胶质瘤患者肿瘤组织中Ldb1表达增高,可能参与了胶质瘤的发生、发展过程。

Objective To investigate the expression of Ldbl in human gliomas and normal brain tissues and evaluate its clinical significance. Methods The level of LLM- domain- binding 1 （ Ldbl ） in brain specimens collected from 46 patients with gliomas, 18 normal controls and 16 benign brain tumors was measured by quantitative real-time PCR. EnVision Immunohistochemistry technique was also applied to detect the protein expression of Ldbl in formalin-fixed paraffin embedded samples of high-grade gliomas. Results Ldbl expression in glioma patients was statistically higher than that in the controls. Compared with that in lowgrade gliomas, Ldbl mRNA levels in high-grade gliomas were significantly increased （P 〈 0.001 ）. Ldbl protein was localized in nucleus and cytoplasmin human high-grade glioma cells. Conclusion The expression of Ldbl is elevated in glioma cells. It may be related to the occurrence and development of glioma.