假耧斗菜中化学成分的研究

Study on chemical constituents of Paraquilegia microphylla

目的 研究假耧斗菜中的化学成分。方法 运用多种方法对其化学成分进行纯化和鉴定,并运用3-（4,5-二甲基吡啶-2）-5-（3-羧基甲氧基苯基）-2-（4-磺苯基）-2-氢-四唑盐（MTS）比色法考察了化合物Ⅰ和Ⅱ的对肺癌细胞H460、人乳腺癌细胞MCF-7和人肝癌细胞Hep G2的体外抗肿瘤活性。结果 共分离得到7个化合物,分别为：Paraquinin A（Ⅰ）、Praquinin B（Ⅱ）、小檗碱（Ⅲ）、Menisdaurin（Ⅳ）、3,4-二甲基苯甲酸（Ⅴ）、对羟基苯甲酸（Ⅵ）、己内酰胺（Ⅶ）,抗肿瘤活性结果显示化合物Ⅰ和Ⅱ均无细胞毒活性。结论化合物Ⅲ～Ⅶ为首次从该植物中分离得到,并首次测试了化合物Ⅰ和Ⅱ的抗肿瘤活性。

Objective To investigate the chemical constituents of Paraquilegia microphytla. Methods The constituents were separated and purified by chromatographic methods, and their structures were elucidated by spectroscopic methods, compounds Ⅰ and Ⅱ were evaluated for their cytotoxicity against human H460 non-small cell lung cancer cells （H460）, the breast cancer cell line MCF-7 （MCF-7） and human hepatocellular cell line Hep G2 （Hep G2） by [3-（4,5- dimethyhhiazol-2-yl）-5-（3-carboxymethoxyphenyl）-2-（4-sulfophenyl）-2H-tetrazolium （MTS） method. Results Seven known compounds were obtained andlidentified as Paraquinin A （ Ⅰ ）, Praquinin B （Ⅱ）, Berberine （Ⅲ）, Menisdaurin （Ⅳ）, 3,4-dimethyl benzoic acid （Ⅴ）, 4-hydroxybenzoic acid （Ⅵ） and caprolactam （Ⅶ）, the results of the cytotoxicity about compounds Ⅰ and Ⅱ were inactive against several human cancer ceils. Conclusion Compounds III-Vl[ are isolated from this plant for the first time, the cytotoxicity of compounds Ⅰ and Ⅱ against several human cancer cells were tested for the first time.