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IL-17A对柯萨奇B3病毒性心肌炎小鼠心肌的影响 被引量:3

Effect of IL-17A on Coxsackie B3 viral myocarditis mice myocardium
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摘要 目的探讨IL-17A对IL-17A基因敲除柯萨奇B3病毒性心肌炎小鼠心肌的影响。方法取15只IL-17A基因敲除型小鼠作为IL-17A基因敲除型组,15只WT小鼠作为对照组,各组小鼠腹腔内注射Nancy株柯萨奇病毒建立病毒性心肌炎小鼠模型,观察两组小鼠的心肌HE染色、心肌组织病理积分、血清细胞因子和心肌组织TGF-β1蛋白水平情况。结果 HE染色结果显示:两组小鼠建模后第7、14、28天时心肌组织均出现炎症反应,IL-17A基因敲除组小鼠各时间点心肌组织的炎症反应均较对照组轻,第14天时,两组小鼠心肌组织炎性反应均最严重,对照组小鼠心肌炎症较IL-17A基因敲除组小鼠严重,出现广泛的炎症细胞浸润,心肌纤维化和心肌细胞坏死比较严重,IL-17A基因敲除组小鼠心肌组织炎症较对照组轻。IL-17A基因敲除组第7、14、28天心肌组织病理积分均低于对照组,差异有统计学意义(P<0.05)。IL-17A基因敲除组小鼠血清INF-γ高于对照组,IL-17A基因敲除组小鼠血清TNF-α、IL-17和IL-6水平低于对照组,差异有统计学意义(P<0.05)。IL-17A基因敲除组第7、14、28天小鼠心肌组织TGF-β1蛋白水平均低于对照组,差异有统计学意义(P<0.05)。结论敲除IL-17A基因能够降低病毒性心肌炎小鼠的心肌炎症,降低心肌组织病理积分,影响血清细胞因子水平,降低心肌组织TGF-β1蛋白水平。 Objective To study the myocardium of IL-17A gene knockout viral myocarditis mice, and explore the effect of IL-17A on Coxsackie B3 viral myocarditis mice myocardium. Methods 15 IL-17A gene knockout mice were selected as IL-17A gene knockout group, 15 WT mice were selected as control group, mice of each group were injected Nancy strain coxsackievirus to establish viral myocarditis mice model, observed myocardial HE staining, myocardial pathological scores, serum cytokines and myocardial tissue TGF-β1 protein level of two groups mice. Results HE staining showed: mice of two groups all showed myocardial inflammation at 7th, 14th, 28th day after modeling, cardiac tissue inflammation reaction of IL-17A gene knockout group mice was lighter than control group, at the 14th day, cardiac tissue inflammatory reaction of two groups mice were the most serious, the control group mice appeared widespread inflarnrnatory cell infiltration, myocardial necrosis and myocardial fibrosis compare serious, cardiac tissue inflammation reaction of IL-17A gene knockout mice lighter than control group. The myocardial pathological scores of IL-17A gene knockout group were lower than those of control group at 7th, 14th, 28 day, the difference was statistically significant (P 〈 0.05). The serum INF-α of IL-17A gene knockout group mice was higher than that of control group, the serum TNF-α, IL-17 and IL-6 levels of IL-17A gene knockout group mice were lower than those of control group, the differences were statistically significant (P 〈 0.05). The myocardial TGF-β1 protein levels of IL-17A gene knockout group mice were lower than control group at 7th, 14th, 28 day, the differences were statistically significant (P 〈 0.05). Condusion IL-17A gene knockout can reduce viral myocarditis mice myocardial inflammation, reduce myocardial histopathologic score, influence serum cytokine levels, reduce myocardial tissue TGF-β1 protein levels.
出处 《中国医药导报》 CAS 2016年第16期23-26,共4页 China Medical Herald
基金 浙江省温岭市科学技术局科技项目(2011WLCB0087)
关键词 IL-17A 病毒性心肌炎 株柯萨奇病毒B3 小鼠 IL-17A Viral myocarditis Strains of Coxsackie virus B3 Mice
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参考文献21

  • 1Patil KG,Salagre SB,Itolikar SM. Left ventricular non-com-paction with viral myocarditis: a rare presentation of a rarerdisease [J], J Assoc Physicians India,2014,62 (3) :261 -263.
  • 2Wang Y,Gao B,Xiong S. Involvement of NLRP3 inflamma-some in CVB3-induced viral myocarditis [J]. Am J Phys-iol Heart Circ Physiol,2014,307(10) :H1438- H1447.
  • 3Jenke A,Holzhauser L,Lobel M,et al. Adiponectin promotescoxsackievirus B3 myocarditis by suppression of acute anti-viral immune responses fj]. Basic Res Cardiol,2014,109(3):408.
  • 4Lee CJ, Huang YC, Yang S,et al. Clinical features of cox-sackievirus A4,B3 and B4 infections in children [J]. PLoSOne,2014,9(2):e87391.
  • 5Xie Y,Li M,Wang X,et al. In vivo delivery of adenoviralvector containing interleukin-17 receptor a reduces car-diac remodeling and improves myocardial function in viralmyocarditis leading to dilated cardiomyopathy [J]. PLoSOne,2013,8(8):e72158.
  • 6Kong Q,Xue Y, Wu W,et al. IL-22 exacerbates the sever-ity of CVB3-induced acute viral myocarditis in IL-17A-deficient mice [J]. Mol Med Rep, 2013,7(4) ; 1329-1335.
  • 7Kishimoto G,Takamatsu N,Ochiai H,et al. Nucleotide dif-ferences of coxsackievirus B3 and chronic myocarditis [J].Heart Vessels,2015,30(1): 126-135.
  • 8Koenig A,Sateriale A,Budd RC,et al. The role of sex dif-ferences in autophagy in the heart during coxsackievirusB3 -inducedmyocarditis [J]. J Cardiovasc Transl Res,2014,7(2):182-191.
  • 9Bao JL,Lin L. MiR-155 and miR-148a reduce cardiacinjury by inhibiting NF -kB pathway during acute vi-ralmyocarditis [J]. Eur Rev Med Pharmacol Sci,2014,18(16):2349-2356.
  • 10Sinnecker D, Laugwitz KL, Moretti A. Extending humaninduced pluripotent stem cell technology to infectiousdiseases:new model forviral myocarditis [J]. Circ Res,2014,115(6);537-539.

二级参考文献32

  • 1LIU P P, MASSON J W. Advances in the understanding of myocarditis [ J ]. Circulation, 2001, 104 (9) : 1076-1082.
  • 2HEYMANS S, PAUSCHINGER M, D E PALMA A, et al. Inhibition of urokinase-type plasminogen activator or matrix metalloproteinases prevents cardiac injury and dysfunction during viral myocarditis [ J ]. Circulation, 2006, 114 (6) : 565- 573.
  • 3IVANOV S, LINDN A. Interleukin-17 as a drug target in human disease[J]. Trends Pharmacol Sci, 2009, 30(2): 95- 103.
  • 4MI S, LI Z, YANG H Z, et al, Blocking IL-17A promotes the resolution of pulmonary inflammation and fibrosis via TGF- betal-dependent and -independent mechanisms [J].J Immunol, 2011, 187(6) : 3003-3014.
  • 5FENG W, LI W, LU W, et al. IL-17 induces myocardial fibrosis and enhances RANKL/OPG and MMP/TIMP signaling in isoproterenol-induced heart failure[J].Exp Mol Pathol, 2009, 87(3) : 212-218.
  • 6FAN Y, WEIFENG W, YULUAN Y, et al. Treatment with a neutralizing anti-murine interleukin-17 antibody after the onset of coxsackievirus b3-induced viral myocarditis reduces myocardium inflammation[J]. Virol J, 2011, 8(1) : 17.
  • 7NAKAE S, KOMIYAMA Y, NAMBU A, et al. Antigen-specific T "cell sensitization is impaired in IL-17-deficient mice,causing suppression of allergic cellular and humoral responses[J]. Immunity, 2002, 17(3) : 375-387.
  • 8QUEREJETA R, VARO N, LOPEZ B, et al. Serum carboxy- terminal propeptide of procollagen type I is a marker of myocardial fibrosis in hypertensive heart disease [J]. Circulation, 2000, 101 (14) : 1729-1735.
  • 9FAIRWEATHER D, ROSE N R. Coxsackievirus-induced myocarditis in mice: a model of autoimmune disease for studying immunotoxicity [J]. Methods, 2007, 41 (1) : 118- 122.
  • 10CAFORIO A L, TONA F, BOTTARO S, et al. Clinical implications of anti-heart autoantibodies in myocarditis and dilated cardiomyopathy[J]. Autoimmunity, 2008, 41 ( 1 ) : 35- 45.

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