自噬在谷胱甘肽保护槟榔碱致肝损害中的作用

Effect of autophagy on glutathione protecting against arecoline-induced hepatic injury

目的探讨自噬在L-谷胱甘肽保护槟榔碱所致肝损害中的作用及机制。方法建立小鼠槟榔碱致肝损害的模型,应用L-谷胱甘肽进行干预后对比观察小鼠的生活状态、肝功能AST、ALT及肝脏形态学变化;采用免疫组化SP法检测肝组织中微管相关蛋白1轻链3(microtubule associated protein 1 light chain 3,LC3)的表达。结果槟榔碱导致小鼠习性变化、体重减轻,AST、ALT水平明显升高,肝细胞水肿及坏死;L-谷胱甘肽能明显改善上述表现。LC3在实验组的表达显著高于正常对照组(P<0.05),且谷胱甘肽与槟榔碱联合应用组显著高于单纯槟榔碱组(P<0.05)。结论 L-谷胱甘肽能有效保护槟榔碱所致的肝损害,自噬活性的提高可能是L-谷胱甘肽发挥护肝作用的重要机制。

Purpose To explore the effect and mechanism of autophagy on L-glutathione protecting against arecoline-induced hepatic damage. Methods By establishing a mice model of liver injury induced by arecoline and application of L-glutathione to intervention, we observed the characteristics of life, level of AST, ALT and pathology of liver. And the expression of microtubule associated protein 1 light chain 3 （LC3） in liver tissue was detected by immunohistochemical method. Results The mice habits changed, weight lost, and AST, ALT levels increased significantly, and the liver cells had edema and necrosis by arecoline. L-glutathione can reverse the a- bove changes significantly. The expression of LC3 in the experimental groups was significantly higher than that in the normal control group （P 〈 0. 05 ） , and that in combined application of glutathione and arecoline was significantly higher than that of simple arecoline group （P 〈 0. 05）. Conclusion L-glutathione can protect the arecoline-induced hepatic injuries effectively. The enhanced activity of autophagy may be an important mechanism for L-glutathione to protect liver.