摘要
目的探讨叶酸-壳聚糖-碳纳米管-顺铂(cisplatin-carbon nanotube-chitosan-folic acid,DDP-SWCNT-CHI-FA)复合物对人卵巢癌细胞的影响。方法制备DDP-SWCNT-CHI-FA复合物,测定复合物中顺铂的药载浓度;采用MTT法检测药物对卵巢癌细胞的作用;应用Western blot法检测SKOV3/DDP细胞中铜转运蛋白-1(human copper transporter-1,h CTR-1)的表达。结果 SWCNT-CHI-FA和FA-CHI携载顺铂的载药率分别为125.7%和46.08%;顺铂和DDP-SWCNT-CHI-FA对卵巢癌细胞株SKOV3的IC50分别为(8.27±0.39)μg/μl和(5.18±0.83)μg/μl,对其耐药株SKOV3/DDP的IC50分别为(37.43±1.24)μg/μl和(28.23±1.17)μg/μl;SKOV3/DDP细胞中DDP-SWCNT-CHI-FA组的h CTR-1蛋白表达水平较顺铂组低。结论制备的DDP-SWCNT-CHI-FA复合物具有高载药率;可以增强顺铂对卵巢癌顺铂耐药细胞的抑制作用。
Purpose To investigate the effects of cisplatin-carbon nanotube-chitosan-folic acid （DDP-SWCNT-CHI-FA） composites on human SKOV3 ovarian cancer cells in vitro. Methods DDP-SWCNT-CHI-FA composites were prepared, The concentration of eisplatin in the DDP-SWCNT-CHI-FA composites were analyzed. The drug effects on SKOV3 cells and SKOV3/DDP cells in vitro were examined by MTT. The human copper transporter-1 （ hCTR-1 ） protein expression in the SKOVJDDP cells were detected by Western blot. Results SWCNT-CHI-FA and FA-CHI carrying eisplatin drug-loading rate were 125.7% and 46.08%. Cisplatin and DDP-SWCNT- CHI-FA ICso of ovarian cancer cell lines SKOV3 were （8.27 ±0. 39）μg/μl and （5. 18 ±0. 83） μg/μl, the IC5o of SKOV3/DDP resistant strains were （37.43 ± 1.24） μg/μl and （28.23 ±1.17 ） μg/μl. The expression level of the hCTR-1 protein in DDP-SWCNT- CHI-FA group was lower than eisplatin group in the SKOV3/DDP cells. Conclusion The DDP-SWCNT-CHI-FA composites have a high cisplatin-load rate and can enhance the inhibitory effect of cisplatin on SKOV3/DDP ovarian cancer cells. The mechanism may be related to down regulate hCTR-1 protein expression.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2016年第6期669-673,共5页
Chinese Journal of Clinical and Experimental Pathology
基金
桂林市人口健康安全技术开发与应用研究项目(20140120-1-11)
