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体外受精中3原核胚胎的发育及可利用价值的探讨 被引量：3

Exploration of the development and utility of tripronuclear embryos after in-vitro fertilization
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摘要 目的:探讨体外受精(IVF)中异常的3原核(PN)胚胎的发育及可利用价值。方法:收集IVF治疗周期中废弃的3PN受精卵204个进行体外培养,观察其发育能力,并与同周期的1 138个2PN受精卵进行比较;采用胚胎植入前遗传学筛查(PGS)技术对由3PN发育成的19枚囊胚进行非整倍体分析。结果:3PN组和2PN组的卵裂率无统计学差异(P>0.05);但3PN组囊胚形成率显著低于2PN组[9.6%(19/97)vs 37.9%(204/342),P<0.01]。整倍体分析显示,10.5%(2/19)的3PN来源的囊胚为正常二倍体核型。结论:3PN受精卵有继续发育能力;囊胚培养和高通量测序可作为有效筛选异常PN受精卵中正常核型胚胎的一种方法。 Objective： To explore the development and utility value of tripronuclear （3PN） embryos after conventional in-vitro fertilization （IVF）. Methods： Totally 204 discarded 3PN zygotes were cultured during IVF and developmental potentiality of 3PN zygotes was compared with bipronuclear （2PN） zygotes. Preimplantation genetic screening （PGS） technology was employed to investigate aneuploid of 19 3PN blastocysts. Results： 3PN and 2PN groups demonstrated equal cleavage rates, but 3PN group showed lower blastocyst formation rate, and some 3PN blastocysts had normal diploid karyotype. Conclusion： Blastocyst culture and high-throughput sequencing （HTS） were effective methods for screening normal karyotype embryos from abnormal pronucleus zygotes.
作者 岳钊平 辛欣 李云芳 万晓静 谢守珍
机构地区 广州军区武汉总医院生殖医学中心
出处 《生殖与避孕》 CAS CSCD 北大核心 2016年第6期516-520,共5页 Reproduction and Contraception
关键词 3原核(PN)胚胎 囊胚 体外培养 高通量测序(HTS) 整倍体分析 tripronuclear （3PN） embryos blastocyst in vitro culture high-throughput sequencing （HTS） euploid analysis
分类号 R714.8 [医药卫生—妇产科学]
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参考文献14

  • 1Feng H, Hershlag A. Fertilization abnormalities following human in vitro fertilization and intracytoplasmic sperm injection. Mierosc Res Tech, 2003, 61(4):358-61.
  • 2Pang MG, Jee BC, Kim SI-I, et al. Chromosomal constitution of embryos derived from Iripronuclear zygotes studied by fluo- rescence in situ hybridization using probes for chromosomes 4, 13, 18, 21, X, and Y. Gynecol Obstet Invest, 2005, 59(1):14-8.
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二级参考文献32

  • 1王仁礼.植入前遗传学诊断[J].生殖与避孕,2004,24(3). 被引量：3
  • 2Egozcue S, Blanco J, Vidal F, et al. Diploid sperm and origin of triploidy. Hum Reprod, 2002, 17(1):5-7.
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生殖与避孕
2016年 第6期

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