应用Alexa Fluor488与BHQ1双标记寡核苷酸荧光探针测量辐射吸收剂量的可行性研究

The feasibility study of radiation absorbed dose measurement using oligonucleotide dually labeled with Alexa Fluor 488 and BHQ1 probe

目的 研究荧光探针Alexa Fluor 488-DNA-BHQ1用于辐射吸收剂量测量的可行性。方法 在寡核苷酸的5＇与3＇端分别标记荧光分子Alexa Fluor 488与其特异性荧光抑制剂BHQ1,制备Alexa Fluor 488-DNA-BHQ1的DNA双标记荧光探针。用X射线照射其水溶液,检测照射后溶液的荧光强度。结果 浓度为0.5~1μmol/L时,该荧光探针对剂量0.1~30 Gy之间线性响应最好（R2=0.99）。在辐照后40~80 min检测探针,荧光强度基本不变。4℃条件下保存受照后的荧光探针的荧光稳定性较好。结论 荧光探针Alexa Fluor 488-DNA-BHQ1可以应用于0.1~30 Gy范围内辐射吸收剂量的测量。

Objective To study the feasibility of measuring radiation absorbed dose with the fluorescent probe Alexa Fluor 488-DNA-BHQ1. Methods An oligonucleotide dually labeled at its 5＇-and 3＇-end with fluorescent molecular Alexa Fluor 488 and specific fluorescence inhibitors BHQ1 was prepared. The Alexa Fluor 488-DNA-BHQ1 aqueous solution was exposed with X-ray and its fluorescence intensity was measured. Results When the concentration of Alexa Fluor 488-DNA-BHQ1 was between 0.5 and 1 μmol/L, the fluorescence intensity of its aqueous solution had excellent linear dose response from 0.1 to 30 Gy （R2=0.99） and it was stably maintained after 40-80 min of irradiation especially at 4℃. Conclusions In the dose range of 0.1-30 Gy, the Alexa Fluor 488-DNA-BHQ1 fluorescent probe can be used to measure radiation absorbed dose.