UPLC法同时测定夏荔芪胶囊中5种活性成分的含量

Simultaneous Determination of Five Active Ingredients in Xialiqi Capsule by UPLC

目的：建立同时测定夏荔芪胶囊中毛蕊异黄酮葡萄糖苷、特女贞苷、盐酸巴马汀、盐酸小檗碱、迷迭香酸含量的方法。方法：采用超高效液相色谱法。色谱柱为Shield RP18，流动相为乙腈-0.2％甲酸水溶液（梯度洗脱），流速为0.2 ml/min，柱温为35℃，检测波长为240 nm，进样量为1μl。结果：毛蕊异黄酮葡萄糖苷、特女贞苷、盐酸巴马汀、盐酸小檗碱、迷迭香酸检测质量浓度线性范围分别为0.619～39.6、1.181～115.6、0.89～57.2、1.16～74、2.47～157.8μg/ml（r均0.9999）；精密度、稳定性、重复性试验的RSD＜3％；加样回收率分别为97.98％～102.44％、97.37％～100.96％、98.53％～102.30％、97.06％～102.21％、100.10％～102.25％，RSD分别为1.49％、1.13％、1.40％、1.46％、0.68％（n＝9）。结论：该方法简单快速、准确性好，可用于控制夏荔芪胶囊的质量。

OBJECTIVE：To establish a method for simultaneous determination of calycosin-7-glucoside,specnuezhenide,ber-berine hydrochloride,palmatine hydrochloride and rosmarinic acid in Xialiqi capsule. METHODS：UPLC was performed on the col-umn of Shield RP18 with mobile phase of nitrile-0.2%formic acid（gradient elution）at a flow rate of 0.2 ml/min,the column tem-perature was 35 ℃,the detection wavelength was 240 nm,and the injection volume was 1 μl. RESULTS：The linear range was 0.619-39.6 μg/ml for calycosin-7-glucoside（r=0.999 9）,1.181-115.6 μg/ml for specnuezhenide（r=0.999 9）,0.89-57.2 μg/ml for berberine hydrochloride（r=0.999 9）,1.16-74 μg/ml for palmatine hydrochloride（r=0.999 9）and 2.47-157.8 μg/ml for rosmarinic acid（r=0.999 9）;RSDs of precision,stability and reproducibility tests were lower than 3%,recoveries were 97.98%-102.44%（RSD=1.49%,n=9）,97.37%-100.96%（RSD=1.13%,n=9）,98.53%-102.30%（RSD=1.40%,n=9）,97.06%-102.21%（RSD=1.46%,n=9）and 100.10%-102.25%（RSD=0.68%,n=9）,respectively. CONCLUSIONS：The method is simple,rapid and reproducible,and can be used for the quality control of Xialiqi capsule.