RP-HPLC法同时测定消炎利胆片中咖啡酸、异夏佛塔苷、夏佛塔苷的含量

Simultaneous Determination of Caffeic Acid,Isoschaftoside and Schaftoside in Xiaoyan Lidan Tablet by RP-HPLC

目的：建立同时测定消炎利胆片中咖啡酸、异夏佛塔苷、夏佛塔苷含量的方法。方法：采用反相高效液相色谱法。色谱柱为Phenomenex C18，流动相为甲醇-0.1％磷酸水溶液（梯度洗脱），流速为0.8 ml/min，检测波长为334 nm，柱温为25℃，进样量为10μl。结果：咖啡酸、异夏佛塔苷、夏佛塔苷的检测进样量线性范围分别为0.10～2.81、0.20～5.63、0.10～2.63μg（r＝0.9999、0.9999、0.9999）；精密度、稳定性、重复性试验的RSD＜3％；加样回收率分别为96.3％～100.4％（RSD＝1.59％，n＝9）、97.3％～101.2％（RSD＝1.28％，n＝9）、96.6％～100.6％（RSD＝1.39％，n＝9）。结论：该方法简单灵敏、准确可靠、重复性好，可用于同时测定消炎利胆片中咖啡酸、异夏佛塔苷、夏佛塔苷的含量。

ABSTARCT OBJECTIVE：To establish a method for the simultaneous determination of caffeic acid,isoschaftoside and schafto-side in Xiaoyan lidan tablet. METHODS：RP-HPLC was performed on the column of Phenomenex C18 with mobile phase of metha-nol- 0.1% phsphate acid（gradient elution）at flow rate of 0.8 ml/min,the detection wavelength was 334 nm,column temperature was 25 ℃,and the injection volume was 10 μl. RESULTS：The linear range was 0.10-2.81 μg for caffeic acid（r=0.999 9）, 0.20-5.63 μg for isoschaftoside（r=0.999 9）and 0.10-2.63 μg for schaftoside（r=0.999 9）;RSDs of precision,stability and repro-ducibility tests were lower than 3%;recoveries were 96.3%-100.4%（RSD=1.59%,n=9）,97.3%-101.2%（RSD=1.28%,n=9） and 96.6%-100.6%（RSD=1.39%,n=9）,respectively. CONCLUSIONS：The method is sensitive,accurate,reliable and reproduc-ible,and can be used for the simultaneous determination of caffeic acid,isoschaftoside and schaftoside in Xiaoyan lidan tablet the quality control of Xiaoyan lidan tablet.