丝胶对2型糖尿病大鼠胰岛素水平的影响

EFFECTS OF SERICIN ON SERUM INSULIN LEVEL OF TYPE 2 DIABETIC RATS

目的:观察丝胶对2型糖尿病模型大鼠血清胰岛素水平的影响。方法:48只雄性SD大鼠随机分为正常对照组、2型糖尿病模型组、丝胶治疗组和二甲双胍阳性对照组4组,每组12只大鼠。采用高脂高糖饲料喂养联合链脲佐菌素(35mg/kg,2次)连续腹腔注射的方法制作2型糖尿病大鼠模型,以空腹血糖≥11.1mmol/L作为成模标准。待模型成功建立后,丝胶组和阳性对照组大鼠分别给予丝胶(2.4g/kg/d)和二甲双胍(55.33mg/kg/d)灌胃35天。分别采用葡萄糖氧化酶法和ELISA法检测各组大鼠的血糖和血清胰岛素水平。结果:模型组大鼠的血糖明显高于正常组大鼠、血清胰岛素水平明显低于正常组大鼠(P<0.05);丝胶治疗组、阳性对照组大鼠的血糖明显低于模型组大鼠,血清胰岛素水平明显高于模型组大鼠(P<0.05)。结论:丝胶可提高2型糖尿病大鼠血清胰岛素水平,这可能是丝胶降血糖作用的机制之一。

Objective： To observe the effects of sericin on serum insulin level of type 2 diabetic rats. Methods： 48 male SD rats were randomly divided into 4 groups with 12 rats in each group： normal control group, type 2 diabetes mellitus model group, sericin treatment group and metformin positive control group. The type 2 diabetes mellitus rats model was induced by high calorie and high sugar diet plus intraperitoneal injection of streptozotocin（35mg/kg）, and the standard for successfully establishing type 2 diabetes model was fasting blood glucose≥11.1mmol/L. Then the rats in sericin treatment group and postitive control group were respectively lavaged with sericin（2.4g/kg/d） and metformin（55.33mg/kg/d） for 35 days. Glucose oxidase method and ELISA were used to measure blood glucose and serum insulin level respectively. Results： The blood glucose of rats in model group increased obviously compared with rats in normal group, serum insulin level decreased obviously（P 0.05）; The blood glucose of rats in sericin treatment group and positive control group declined obviously compared with rats in model group, serum insulin level rised obviously（P 0.05）. Conclusions： Sericin can increase the serum insulin level of diabetic rats, this maybe one of the mechanisms that sericin decreasing blood glucose.