滚筒式点样仪生物芯片制备效率的研究

Study on the work efficiency of the roller biochip microarrayer

目的描述滚筒式点样仪制备生物芯片的两种方法以及制备周期的计算,分析比较两种点样方法的点样效率,为提高生物芯片制备效率提供理论依据。方法传统的静态点样方法中,为避免因滚筒频繁启停所造成的过冲或振动而采用了一种S形加减速方式。连续动态点样方法中,用遗传算法求得滚筒转速和点样针单圈喷点次数的最优解,计算出点样过程中各个阶段所需的时间,得出生物芯片制备周期,最后用实验验证了计算的准确性。结果传统的静态点样方式生物芯片制备周期为1173 s,连续动态点样方式制备周期为846 s,效率提高了27.8%。结论连续动态点样方式比传统的静态点样方式的生物芯片制备效率更高,更加符合行业要求。

Objective This paper describes two methods of biochip preparation by roller biochip microarrayer and the calculation method of production cycle. We analyze and compare the work efficiency of the two methods in order to provide theoretical basis for improving the preparation efficiency of biological chip. Methods The traditional static spotting method adopts a new S curve acceleration and deceleration control method to avoid the overshoot or the vibration caused by frequent start-stop. The continuous dynamic spotting method adopts the genetic algorithm to solve the optimal solutions of the roller＇s speed and the spotting times in each lap. We calculate the production cycle of biochip by calculating the time in each stage and verify the calculation accuracy by the experiment. Results The production cycle of biochip by the traditional static spotting method is 1173 seconds,and the production cycle of biochip by the continuous dynamic spotting method is 846 seconds. The efficiency increases by 27. 8%. Conclusions The continuous dynamic spotting method is much more effective,more efficient and more conform to the requirements of the industry than the traditional static spotting method.