摘要
【目的】评价日粮中不同水平亮氨酸对繁殖期意大利蜜蜂Apis mellifera ligustica蜂群群势及工蜂发育的影响。【方法】选取重庆荣昌本地繁殖期意大利蜜蜂21群,随机分为7组,分别饲喂添加0,15.30,17.50,26.25,35.00,43.75和52.5 g/kg亮氨酸的试验日粮,每12 d测定各组蜂群群势、封盖子量、蜂群取食量和营造王台情况,24 d后测定蜂群采集行为、工蜂初生重、工蜂虫体蛋白质含量及工蜂寿命。【结果】当亮氨酸添加水平为43.75和52.50 g/kg时能够显著提高繁殖期意大利蜜蜂的蜂群群势、封盖子量、蜂群取食量及采集行为(P<0.05)。与空白对照组相比,基础日粮组及各亮氨酸添加组的新出房蜂和6日龄幼虫体蛋白质含量较高(P<0.05);各亮氨酸添加组的工蜂初生重显著高于空白组(P<0.05);不同亮氨酸添加水平对意大利蜜蜂工蜂寿命影响较小。此外,亮氨酸添加水平26.25,35.00和43.75 g/kg能够在饲喂0-24 d时促进蜂群营造自然王台,刺激蜂群分蜂。【结论】配方日粮中43.75和52.5 g/kg的亮氨酸能够显著促进繁殖期意大利蜜蜂蜂群发展,其中43.75 g/kg的亮氨酸饲喂量还能刺激蜂群营造自然王台,激发蜂群分蜂。
【Aim】This study aims to assess the effects of different leucine levels on the colony and worker development of Apis mellifera ligustica in breeding season. 【Methods】Twenty-one colonies of A.mellifera ligustica in breeding season were obtained from Rongchang,Chongqing,and ranolomly divided into seven groups and fed with diets containing 0,15. 30,17. 50,26. 25,35. 00,43. 75 and 52. 5 g/kg leucine,respectively. During the experiment,the colony population,amount of sealed brood,feeding amount,and queen cell building were recorded every 12 days. The foraging activity,weight of newly emerged adults,protein content and longevity of workers were detected after 24 days. 【Results】The colony population,amount of sealed brood,feeding amount and foraging activity of the groups fed with diets containing 43. 75 and 52. 50 g/kg leucine were significantly higher than those of other groups( P 〈0. 05). The protein contents in 6-day-old larvae and newly emerged workers of the control group were lower than those in the groups feeding diets containing leucine( P 〈0. 05). The weight of newly emerged workers of the control group was significantly lower than that of the groups feeding diets containing leucine( P 〈0. 05). There was no significant difference in the worker longevity among all groups. Building of queen cells was found in the groups fed with diets containing 26. 25,35. 00 and 43. 75 g/kg leucine,respectively,in 0- 24 d. 【Conclusion 】 Adding 43. 75 and 52. 50 g/kg leucine to the diet can significantly promote the development of A. mellifera ligustica colony in breeding season,and 43. 75 g/kg leucine in the diet also stimulates colonies to build queen cells.
出处
《昆虫学报》
CAS
CSCD
北大核心
2016年第5期500-508,共9页
Acta Entomologica Sinica
基金
国家现代农业(蜜蜂)产业技术体系建设专项(CARS-45-SYZ15)
重庆市基本科研业务费基础研究项目(2014cstc-jbky-00110)
重庆市基本科研业务费基础研究项目(15444)
关键词
意大利蜜蜂
亮氨酸
蜂群
工蜂
营养需要
营造王台
Apis mellifera ligustica
leucine
colony
worker
nutrient requirement
queen cell building