HPLC法测定丹参配方颗粒丹参酮ⅡA含量

Determination of Tanshinone ⅡA in Salvia Miltiorrhiza Granules by HPLC

目的：利用HPLC法对丹参配方颗粒中丹参酮ⅡA的实际含量进行精确测定.实验条件：选用高效液相色谱法测定,分离条件为Agilent Zorbax SB-C18色谱柱（250mm×4.6mm,5μm）,甲醇-水（75：25）为流动相,流速1.0 m L/min,检测波长为270nm.结果：丹参酮ⅡA的线性范围为8.03~41.0μg·m L-1（r=0.999 2）,平均加样回收率为97.3%（RSD=1.04%）.结论：HPLC法不仅便于操作、且灵敏度强,在调控丹参配方颗粒含量方面发挥相关作用.

Objective： In order to establish an accurate and practical HPLC method for determinating the math of Tanshinone IIA in Salvia Miltiorrhiza Granules the HPLC methocl is used. Methods： Chromatographic separation was operated on an Agilent Zorbax SB- Cl8 column（ 250 mm × 4. 6mm,5μm） with mobile phase of methanol（ It＇s ratio IS 75： 25）,water solution at a flow rate of 1. 0 m L / min and a column temperature of 22℃. The eluted analytes were detected by on- 1ine UV detector whose wavelength was set at 270 nm. Results： The calibration curves of Tanshinone IIA was linear over the ranges of 8. 03 ~ 41. 0 μg /m L- 1（ r = 0. 999 2）,and the average recoveries was 97. 3%（ RSD = 1. 41%）. Conclusion： The established method is simple,rapid,reliable and can be used for drug contro1. It is meaningful for using in Clinical practice.