TGF-β1参与四氯化碳诱导小鼠急性肝损伤

Involvement of TGF-β1 in carbon tetrachloride-induced acute liver injury in mouse

目的探讨转化生长因子β1(TGF-β1)是否参与四氯化碳(CCl4)诱导的小鼠急性化学性肝损伤过程。方法采用腹腔注射CCl4诱导小鼠急性肝损伤,ELISA法检测血清TGF-β1水平,RT-PCR检测TGF-β信号分子Smad2、3的表达。结果与橄榄油对照小鼠比较,CCl4处理1d及3d的急性肝损伤小鼠不仅血清转氨酶ALT与AST水平升高,血清TGF-β1水平也明显升高,差异有统计学意义(P<0.01);RT-PCR结果显示,CCl4处理小鼠肝组织TGF-β1 mRNA表达水平明显高于橄榄油对照小鼠,其受体TβRII及信号蛋白Smad2与Smad3 mRNA也表达升高。结论 CCl4诱导小鼠急性肝损伤时TGF-β1及其信号分子Smad2、3表达增加,提示TGF-β1参与CCl4诱导小鼠肝损伤过程。

Objective To investigate whether transforming growth factor-beta 1 （TGF-β1） is involved in the process of carbon tetrachloride （ CC14 ） -induced acute chemical liver injury. Methods Acute liver injury in mouse was induced by intraperitoneal injection of CC14. The level of serum TGF-β1 in mouse was detected with enzyme-linkde im- munosorbent assay（ELISA）. The expression of TGF-β1 signaling molecules Smad2,3 mRNA was examined with reverse transcriptase PCR （RT-PCR）. Results Both levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST） and TGF-β1 in serum of mouse increased significantly at 1st and 3rd date after CC14 treatment （P 〈 0. 01 for all）. RT-PCR results showed that the expression of TGF-β1 mRNA in liver was up-regulated significantly in CCla-treated mouse compared with that in the control mouse treated with olive oil, and the expressions of TI3RII, signaling molecules Smad2,3 mRNA were also obviously up-regulated. Conclusion Levels of TGF-β1 and signaling molecules Smad2, Smad3 increase during CCl4-induced acute chemical liver injury in mouse, which suggest that TGF-β1 is involved in the process of CCI4-induced acute chemical liver injury.