脑星型细胞瘤中CD105、SKP2表达及临床意义

The expression of CD105 and SKP2 in astrocytoma of brains and its clinical significance

目的探讨细胞膜糖蛋白(CD105)和S期激酶相关蛋白2(SKP2)在脑星型细胞肿瘤组织中的表达及其临床意义。方法应用免疫组化方法检测不同级别120例脑中枢神经系统星型细胞瘤组织与25例正常人脑组织中CD105,SKP2蛋白的表达情况及相关性。结果 CD105蛋白标记的MVD在正常脑组织不表达,在各级别肿瘤细胞中表达阳性,并有逐级升高的趋势,各组之间的差异与病理分级密切相关(P<0.01)。Skp2在正常脑组织基本不表达,在肿瘤细胞中表达阳性,随病理分级逐渐升高,各组比较差异有统计学意义(P<0.01)。二者表达水平呈正相关。SKP2在肿瘤组织中的血管内皮细胞及血管周围也有阳性表达。结论 CD105,SKP2表达阳性率都随肿瘤恶性进展增强,SKP2在血管内皮细胞及周围也有阳性表达;提示在脑中枢神经系统星型细胞瘤中,二者的表达变化与肿瘤的恶性发展、血管生成方面有密切相关,有可能有协同作用。

Objective This paper aims to find the expression and clinical significance of Endoglin （CD105） and S-phase kinase-associated protein 2 （Skp2） in astrocytoma of brains. Methods Immunohistoehemistry was used to detect CD105 and SKP2 protein expression and the correlation in different levels of 120 human astrocytoma tissues and 25 normal brain tissues. Results Mierovessel density （MVD） marked by CD105 protein did not express in normal brain tissue but it was expressed positively at all levels of astroeytoma. The positive percentage was gradually increased with the pathological grade and they were also closely correlated （P 〈 0.01 ）. Skp2 expression in normal brain tissue was not obvious. The expression in astrocytoma tissues was also gradually increased with pathological grade and they were also correlated （P 〈 0.05 ） with significant statistical differences （ P 〈 0.01 ）. Skp2 also expressed positive in tumor tissue vascular endothelial cells and perivascular tissnes. Conclusion CD105 and SKP2 expression rates are enhanced with the malignant progression of tumors, and vascular endothelial cells are positive expressed. It prompts that the expression changes of CD105 and SKP2 in the human brain astrocytoma are closely related with the development of malignant tumors and angiogenesis. There may be a synergistic effect.