响应面法优化白背三七多糖的提取工艺

Optimization of microwave extraction condition of Gynura divaricata polysaccharide by response surface analysis

以白背三七为原料,采用微波辅助法提取白背三七多糖。在单因素实验的基础上,采用响应面法优化白背三七多糖的提取工艺。实验结果表明,影响微波辅助提取白背三七多糖的主要因素依次为:液料比、微波时间、微波功率,其中料液比对提取率的影响达到了极其显著水平。白背三七多糖提取的最佳工艺参数为:液料比45∶1(m L/g)、微波功率390 W、微波时间22 min。在此条件下,白背三七多糖的提取率为15.36%,与模型预测值接近。因此,采用响应曲面法优化得到的工艺条件具有一定的实际应用价值。

Gynura divaricata polysaccharide was extracted from Gynura divaricata with the method of microwaveassisted extraction. Based on the results of single factor experiments, the extraction process was optimized by response surface analysis. The results indicated that the influencing factors during extraction were： liquid-solid ratio, microwave time, and microwave power, among which the influence of liquid-solid ratio was the most significant. The optimal conditions were as follows： liquid-solid ratio of 45∶1（mL/g）, microwave power of 390 W, microwave time of 22 min. Under optimal extraction conditions, the extraction rate of Gynura divaricata polysaccharide was 15. 36%, which was pretty close to the predicted result. The optimized response surface methodology had some practical value for the process.