基于DNA条形码(ITS2)的中药材防风与其混伪品的鉴定

Identification of Saposhnikovia divaricata and its adulterants based on DNA barcode(ITS2)

目的通过ITS2序列分析,对防风及其易混伪品进行鉴定,确保用药安全。方法从Gen Bank数据库下载中药材防风与其混伪品的DNA条形码ITS2序列,利用MEGA 6.06软件进行比对分析,计算GC含量及变异位点、种内、种间遗传距离,并构建NJ系统发育树,应用ITS2数据库网站预测其ITS2二级结构。结果序列分析与遗传距离结果显示,防风与其混伪品存在明显差异,基于ITS2序列的NJ树及其ITS2二级结构均可以将防风与其混伪品进行区分。结论 ITS2能够准确鉴定防风及其易混伪品,可用于中药材的快速鉴别。

Objective To identify Saposhnikovia divaricata and its adulterants by using ITS2 sequence analysis and to secure medication safety. Methods ITS2 sequences of Saposhnikovia divaricata and its adulterants were downloaded from Gen Bank,MEGA 6.06 software was used for sequence analysis to summarize GC content,variation sites,estimate intra-and inter-specific genetic distances,and built NJ trees.Moreover,the secondary structure of ITS2 was predicted by using the ITS2 database websites. Results ITS2 sequence analysis and genetic distances calculation showed significant difference in the samples.Further more,NJ tree and secondary structure results could distinctly differentiate quality product and adulterants. Conclusion ITS2 can effectively identify Saposhnikovia divaricata and its adulterants,as a result,it can be used for rapid identification of Chinese medicinal materials.