不同人工诱导方法产生龙血竭的镇痛、抗炎效果的比较

Comparison of analgesic and anti-Inflammatory effect of Resina Draconis generated by different artificial induction methods

目的研究不同人工诱导方法产生的龙血竭镇痛、抗炎效果。方法实验资料来源于广西中医药大学制药厂实验室,收集时间为2015年12月,镇痛实验采用扭体实验方法,将80只小鼠随机分为生理盐水组,阿司匹林组(0.5 g/kg),自然产龙血竭小、大剂量组(1 g/kg和2 g/kg),生物诱导龙血竭小、大剂量组(1 g/kg和2 g/kg),化学诱导龙血竭小、大剂量组(1 g/kg和2 g/kg),每组10只,各组按0.1 ml/10 g剂量灌胃给药,1次/d,连续3 d,观察各组药物对化学刺激痛反应的影响(扭体次数、扭体潜伏期、抑制率);抗炎实验为小鼠50只,随机分为5组,每组10只,即空白对照组(给予同体积蒸馏水)、阿司匹林阳性对照组(0.2 g/kg)、自然产龙血竭组(2 g/kg)、生物诱导龙血竭组(2 g/kg)、化学诱导龙血竭组(2 g/kg),采用二甲苯致小鼠耳肿胀法,连续灌胃给药7 d,1次/d。于末次给药后1 h,在小鼠右耳涂20μl的二甲苯致炎。1 h后处死动物,剪下双耳,用6 mm打孔器切下双耳片称重,以右耳重量减去左耳重,观察其抗炎效果(耳肿胀程度)。结果扭体实验:自然产龙血竭大、小剂量组,生物诱导龙血竭大、小剂量组,化学诱导龙血竭大、小剂量组,阿司匹林组的扭体次数分别为(9.01±6.87)、(10.05±7.87)、(10.51±6.37)、(11.05±7.57)、(10.39±7.35)、(10.15±7.12)、(10.00±4.57)次,与生理盐水组[(21.10±10.62)次]比较,差异有统计学意义(P<0.01),提示其能明显抑制小鼠的扭体次数;自然产龙血竭大、小剂量组,生物诱导龙血竭大、小剂量组扭体镇痛作用与阿司匹林组相似(P>0.05);生物诱导龙血竭剂量组,化学诱导龙血竭剂量组与自然产龙血竭剂量组扭体次数比较,差异无统计学意义(P>0.05)。抗炎实验:自然产龙血竭剂量组、生物诱导龙血竭剂量组、化学诱导龙血竭剂量组、阿司匹林组、空白对照组耳肿胀度分别为(7.8±2.3)、(7.3±2.6)、(7.5±2.8)、(7.0±2.2)、(11.2±2.4)mg,龙血竭给药各组、阿司匹林组耳肿胀度与空白对照组比较差异有统计学意义(P<0.05);生物诱导龙血竭剂量组,化学诱导龙血竭剂量组耳肿胀度与自然产龙血竭剂量组比较,差异无统计学意义(P>0.05)。结论不同人工诱导产龙血竭镇痛、抗炎效果差异不明显。

Objective To make an investigation on the comparison of analgesic and anti-inflammatory effects of Resina Draconis generated by different artificial induction methods. Methods The experimental data was derived from the laboratory of pharmaceutical factory of Guangxi University of TCM,which was collected in December 2015.The writhing test method was adopted in the analgesic test that recruited and divided 80 mice into 8 groups randomly and evenly with 10 mice in each group,including Saline Group,Aspirin Group（0.5 g/kg）,Large-Dosage Natural Group and SmallDosage Natural Group（naturally-produced Resina Draconis with small dosage and with large dosage,1 g/kg and 2 g/kg）,Large-Dosage Biological Group and Small-Dosage Biological Group（biological-induction-produced Resina Draconis with small dosage and with large dosage,1 g/kg and 2 g/kg）,Large-Dosage Chemical Group and Small-Dosage Chemical Group（chemicalinduction-produced Resina Draconis with small dosage and with large dosage,1 g/kg and 2 g/kg）.The drug was administered orally to mice in each group at the dose of 0.1 ml/10 g,1 time a day,for 3 consecutive days,the effect of each drug on chemically-stimulated pain reactions（the number of writhing times,writhing latency and the inhibition rate） were observed.As for the anti-inflammatory test,50 mice were recruited and divided into 5 groups randomly and evenly with 10 mice in each group,including Control Group（administered with the same volume of distilled water）,Aspirin Positive Control Group（0.2 g/kg）,Natural Group（naturally-produced Resina Draconis,2 g/kg）,Biological Group（biological-induction-produced Resina Draconis,2 g/kg） and Chemical Group（chemical-induction-produced Resina Draconis,2 g/kg）.The drugs were continuously and intragastrically administered to all the mice one time a day,for 7 days.The right ears of all the mice were coated with 20 μl xylene 1 hour after the last administration to make all the mice′ s right ears swelling.All the mice were killed 1 hour later and their ears were cut off.Then,the ear flaps were cut off with a 6 mm punch and weighed to calculate the weight gaps between the right ear and the left ear and observe the anti-inflammatory effect（the swelling degree of ears）. Results According to the writhing test results,the number of writhing times in Large-Dosage Natural Group and Small-Dosage Natural Group,Large-Dosage Biological Group and Small-Dosage Biological Group,Large-Dosage Chemical Group and Small-Dosage Chemical Group as well as Aspirin Group were（9.01±6.87）,（10.05±7.87）,（10.51±6.37）,（11.05±7.57）,（10.39±7.35）,（10.15±7.12）,（10.00±4.57） times versus Saline Group′s of（21.10±10.62） times,which showed obvious inhibitory effects on the number of writhing times（P〈0.01） in all the groups except Saline Group.Large-Dosage Natural Group,Small-Dosage Natural Group,Large-Dosage Biological Group,Small-Dosage Biological Group,Large-Dosage Chemical Group and Small-Dosage Chemical Group demonstrated similarities with Aspirin Group in the analgesic effects（P〈0.05）.No significant difference was seen between the writhing frequency of Large-Dosage Natural Group,SmallDosage Natural Group,Large-Dosage Biological Group,Small-Dosage Biological Group,Large-Dosage Chemical Group and Small-Dosage Chemical Group（P 0.05）.As for the anti-inflammatory experiment,the swelling degree of ears in Natural Group,Biological Group,Chemical Group,Aspirin Positive Control Group and Control Group were（7.8±2.3）,（7.3±2.6）,（7.5 ±2.8）,（7.0 ±2.2）,（11.2 ±2.4） mg respectively,with the existence of significant difference between Control Group and the other four groups（P〈0.05）.There was no significant difference between the anti-inflammatory effect of Natural Group,Biological Group and Chemical Group（P 0.05）. Conclusion Resina Draconis generated by different artificial induction methods proves itself with no significant difference in anti-inflammatory and analgesic effects.