SD大鼠40%酒精生理盐水合剂静脉麻醉的可行性探讨

Feasibility of intravenous anesthesia with 40% alcohol saline mixture in SD rats

目的研究SD大鼠40%酒精生理盐水合剂静脉麻醉的可行性,观察其急性中毒反应,找出麻醉最佳剂量。方法将80只SD大鼠分成8组,一个空白对照组及40%酒精生理盐水合剂7个剂量组（14、16、18、20、22、24、26 ml/kg）,每组10只。对照组经尾静脉注射生理盐水,剂量组分别经尾静脉注射不同剂量的40%酒精生理盐水合剂。观察大鼠麻醉过程中中毒反应,记录相关数据。根据结果,最佳剂量组补充大鼠20只重复实验。结果40%酒精生理盐水合剂20 ml/kg剂量组达到酒精中毒（麻醉状态）的有效率最高（83.3%）,麻醉时间长（5.28±1.43）h;24、26 ml/kg剂量组死亡率高（40%~60%）。结论 40%酒精生理盐水合剂静脉麻醉安全、可行,麻醉诱导期短、麻醉时间长,适用于中浅麻醉,20 ml/kg剂量可以作为SD大鼠酒精麻醉的参考剂量。

Objective To study the feasibility of intravenous anesthesia with 40% alcohol saline mixture and its optimal dose and observe the acute alcohol toxic reactions of SD rats. Methods Eighty SD rats were divided into eight groups（ n =10 each） ： control group and seven different doses of 40% alcohol saline mixture（ 14,16,18,20,22,24,26 ml / kg） groups（ alcohol groups）. Normal saline was given via tail vein in control group,and different doses of 40% alcohol saline mixture were injected via tail vein in alcohol groups. The alcohol toxic reactions of rats in anaesthetic process were observed. Based on the preliminary experiment results,20 rats were supplemented to replicate experiments of the optimum dose. Results In alcohol group of 20 ml / kg dose,the effective rate was the highest（ 83. 3%）,and the duration of anesthesia was the longest（ 5. 28 ± 1. 43） hours. The mortality was higher in alcohol groups of 24 and 26 ml/kg dose（ 40%- 60%）. Conclusions Intravenous anesthesia with 40% alcohol saline mixture has the advantages of safe,feasible,short anesthesia induction period and long duration of anesthesia. It is suitable for middle and superficial anesthesia. The 20 ml / kg dose of 40% alcohol saline mixture can be used as a referential dose for alcohol anesthesia in rats.