雷帕霉素干预急性肺损伤SD大鼠NF-κB p65和STAT-1基因表达研究

The Influence of Rapamycin on NF-κB p65 and STAT-1 Expression in Rats with Acute Lung Injury

目的研究雷帕霉素对输血相关急性肺损伤(TRALI)和急性胰腺炎相关性肺损伤(APALI)的SD大鼠肺组织核转录因子p65(NF-κB p65)和信号转导与转录激活子-1(STAT-1)基因相对表达量的影响。方法将SD大鼠60只随机分成6组,分别为TRALI组、APALI组、雷帕霉素干预TRALI组、雷帕霉素干预APALI组、雷帕霉素干预对照组、正常对照组,每组10只。应用实时荧光定量RT-PCR检测大鼠肺组织NF-κB p65和STAT-1基因相对表达量,分析雷帕霉素对TRALI、APALI在mTOR相关细胞凋亡过程的干预作用。结果相比正常对照组,TRALI及APALI组大鼠肺组织NF-κB p65和STAT-1基因相对表达量均有不同程度下降(P<0.05),且APALI组下降程度比TRALI组更明显(P<0.01);雷帕霉素干预后,TRALI组NF-κB p65和STAT-1基因相对表达量与TRALI组比较均进一步下降(P<0.01),而APALI组和对照组下降则不明显或无变化(P>0.05)。结论应用雷帕霉素干预治疗TRALI大鼠可能会进一步加重其肺损伤的程度,而雷帕霉素对APAL大鼠则影响作用有限。

Objective To study the NF- κB p65 and STAT-1 expression in Sprague Dawley rats with transfusion- related acute lung injury or acute pancreatitis associated lung injury. Method Sixty SD rats were randomly divided into TRALI group, APALI group, control group, rapamycin treated TRALI group, rapamycin treated APALI group, and rapamycin treated control group, with 10 animals in each. Gene expression ofNF-κB p65 and STAT-1 was detected by qRT-PCR. The method of 2-AAct was used to calculate the relative qualification of target genes. Result The expression of NF- κ B p65 and STAT-1 in TRALI and APALI.groups was found to be down-regulated （P〈0..05） , and APALI group decreased more thanTRALI group （P〈0.01） . The gene expression of NF-κ B p65 and STAT-1 in rapamycin traeted TRALI group decreased more than in TRALI group （P〈0.01） , while rapamycin intervention APALI group did not decrease significantly compared to APALI group （P〉0.05） . Conclusion Rapamycin might deteriorate pathological process of the lung injury in TRALI, but have limited effects on APALI.