41℃及43℃温热疗联合聚乙二醇化脂质体阿霉素治疗兔VX2肿瘤的疗效对比研究

Comparative study on efficacy of 41 ℃ or 43 ℃ mild hyperthermia with pegylated liposomal doxorubicin for treatment of rabbit VX2 tumor

目的探讨41℃及43℃温热疗(MH)触发聚乙二醇化脂质体阿霉素(PLD)释放阿霉素治疗兔VX2肿瘤的疗效差异。方法 12只VX2移植瘤兔随机分为2组:PLD+41℃MH组(n=6)和PLD+43℃MH组(n=6)。为达到完全的肿瘤缓解,进行多次高剂量耳缘静脉推注给药(5 mg/kg,每周1次,共治疗3次),使用嵌入式超声温热治疗仪对肿瘤部位进行30 min局部MH。结果在观察终点,PLD+41℃MH组血管形成指数(VI)、瘤体质量、外周血谷草转氨酶(GOT)、乳酸脱氢酶(LDH)、肌酸激酶(CPK)值均明显低于PLD+43℃MH组。结论同43℃下30 min MH相比,41℃下30 min MH联合PLD可以明显提高抗肿瘤疗效,降低系统毒性。

Objective To investigate the difference in efficacy between doxorubicin released by 41 ℃ and 43 ℃mild hyperthermia（MH） triggered-pegylated liposomal doxorubicin（PLD） for the treatment of rabbit VX2 tumor.Methods Twelve rabbits with VX2 tumor transplanted into the superficial muscle of the hind limb were randomly assigned to 2 groups,i.e.the 41 ℃ MH ＋ PLD group（n= 6） and the 43 ℃ MH ＋PLD group（n=6）.To achieve complete tumor remission,multiple high-dose administrations（5 mg/kg,once per week for3 weeks） were given by intravenous injection.An embedded ultrasound tumor hyperthermia instrument was used to perform local M H at tumor sites for 30 min.Results At the endpoint,the 41 ℃ MH ＋ PLD group had lower vascularization index,tumor weight,and levels of GOT,LDH and CPK in peripheral blood as compared with the 43 ℃ MH ＋PLD group.Conclusion In comparison with 43 ℃ MH for 30 min,41 ℃MH for 30 min plus PLD can significantly improve the antitumor efficacy,and reduce the systemic toxicity.