左归丸、右归丸对去卵巢骨质疏松大鼠神经肽CGRP、SP、VIP、NPY的影响

Effect of Zuogui and Yougui pills on neuropeptide CGRP,SP,VIP,and NPY in osteoporotic rats after ovariectomy

目的观察补肾药左归丸、右归丸对去卵巢骨质疏松大鼠神经肽的影响,阐明补肾法治疗骨质疏松症的神经调节机制。方法建立去卵巢骨质疏松模型,分别采用ELISA、免疫组化以及PCR观察补肾药干预后骨组织及骨髓间充质干细胞中神经肽CGRP、SP、VIP、NPY的变化。结果切除卵巢后大鼠骨组织及骨髓间充质干细胞中神经肽CGRP、SP、VIP、NPY的水平明显下降,补肾药干预后CGRP、SP、VIP、NPY的水平明显上升,但仍低于假手术组。结论补肾药可以提高骨质疏松大鼠神经肽CGRP、SP、VIP、NPY,这可能是补肾药治疗骨质疏松症的机制之一。

Objective To observe the effect of Zuogui and Yougui pills on the neuropeptides in ovariectomy-induced osteoporotic rats,and to clarify the neural regulation mechanism of kidney tonic medicine in the treatment of osteoporosis. Methods Osteoporosis model was established by ovariectomy. The change of neuropeptides CGRP,SP,VIP,and NPY in the bone and bone marrowmesenchymal stem cells was observed using ELISA,immunohistochemistry,and PCR method after kidney tonic medicine intervention. Results After ovariectomy,the levels of neuropeptides CGRP,SP,VIP,and NPY in the bone tissue and bone marrowmesenchymal stem cells declined obviously. After the intervention of kidney tonic medicine,the levels of CGRP,SP,VIP,and NPY increased obviously,but were still lower than those in sham operation group. Conclusion Kidney tonic medicine improves neuropeptides CGRP,SP,VIP,and NPY in osteoporotic rats. It may be one of the mechanisms of treating osteoporosis.