^(32)P-磷酸铬-聚L-乳酸缓释粒子植入治疗荷人前列腺癌裸鼠移植瘤的实验观察

Experimental observation on therapeutic effect of ^(32)P-chromic-poly L-lactic acid sustained-release particles in nude mice with human prostatic cancer xenografts

目的观察^(32)P-磷酸铬-聚L-乳酸(^(32) P-CP-PLLA)粒子植入及^(32) P-CP注射后对荷人前列腺癌裸鼠移植瘤的治疗效应。方法建立雄性BALB/c裸小鼠荷人前列腺癌细胞株PC-3M皮下移植瘤模型,随机分为三组,每组15只。设为对照组、^(32) P-CP(胶体组)和^(32) P-CP-PLLA(粒子组)。每组各5只裸鼠,每4天测量肿瘤体积,第28天处死小鼠,测量瘤体质量、微血管密度(MVD)和瘤内残余放射性活度。另每组各10只裸鼠定期监测生命指征,采用Kalplan-Meier曲线分析生存情况。结果给药后第28天,粒子组瘤内剩余活度高于胶体组[(1.74±0.^(32))MBq vs.(1.01±0.43)MBq](P<0.01)。粒子组抑瘤率高于胶体组[(59.24±5.68)%vs.(49.52±6.35)%](P<0.01)。粒子组的MVD低于胶体组和对照组(^(32).24±10.07vs.43.15±11.06和62.71±8.21)(P<0.01)。结论 ^(32)P-CP-PLLA粒子植入可以抑制肿瘤生长和血管生成。

Objective To investigate the therapeutic effect of ^32P-chromic-poly L-lactic acid（^32P-CP-PLLA）sustained-release particles in nude mice with human prostatic cancer xenografts.Methods Animal models with human prostatic cancer xenografts were established in 45 male BALB/c nude mice,which were equally randomized into three groups of A（treated with ^32P-CP-PLLA particles）,B（treated with ^32P-chromic phosphate colloid）and C（model control）.The volume of xenografts was measured every 4days in 5mice from each group,which were killed on the 28 th day for the determination of the size,microvessel density（MVD）and residual radioactivity of the xenografts.In another ten mice of each group,vital signs were observed regularly and survival state was analyzed with Kalplan-Meier curve.Results The residual radioactivity of the xenografts on the 28 th day was higher in group A than that in group B[（1.74±0.^32）MBq vs.（1.01±0.43）MBq]（P〈0.01）.The tumor-inhibition rate was higher in group A than that in group B[（59.24±5.68）%vs.（49.52±6.35）%]（P〈0.01）.MVD was lower in group A than that in groups of B and C（^32.24±10.07 vs.43.15±11.06 and 62.71±8.21）（P〈0.01）.Conclusion Implantation of ^32P-CP-PLLA can effectively supress the growth and angiogenesis of human prostatic cancer xenografts in mice model.