姜黄素对大黄鱼组织中磷酸酶活力及血清中细胞因子含量的影响

Effects of Dietary Curcumin on Phosphatase Activity and the Content of Serum Cytokines in Pseudosciaena crocea

为促进大黄鱼免疫增强剂的开发利用,以大黄鱼为研究对象,在饵料中添加不同剂量的姜黄素,研究其对大黄鱼组织中磷酸酶活力及血清中细胞因子含量的影响。试验设置3个试验组(D1-100 mg/kg、D2-150 mg/kg、D3-300 mg/kg)和1个对照组,并分别在第30天、45天取样并测定组织中磷酸酶活力和血清中细胞因子的含量。结果显示:(1)与对照组相比,当姜黄素的添加量为100 mg/kg时,肝脏、前肠中的ACP活力及鳃、胃、肌肉、肝脏中的AKP活力显著提高(P<0.05)。添加量为150 mg/kg时,肌肉、肝脏、前肠中的ACP活力显著提高,鳃、胃、肌肉、肝脏及前肠中的AKP活力显著提高。添加量为300 mg/kg时,除后肠中的ACP、AKP活力及中肠中的AKP活力无显著影响外(P>0.05),其余各组织中的磷酸酶活力均显著提高。(2)与对照组相比,姜黄素添加量为100 mg/kg时,仅TNF-α含量显著升高。添加量为150 mg/kg或300 mg/kg时,GM-CSF、IL-2及TNF-α含量均显著提高。3个试验组IFN-γ含量虽与对照组间无显著差异,但2段时间内均呈下降的趋势。综合考虑,以300 mg/kg姜黄素的添加量对大黄鱼各组织中磷酸酶活力及血清中细胞因子的含量的影响效果最佳。

In order to promote the development of immunopotentiators of Pseudosciaena crocea,the effects of dietary curcumin on acid phosphatase（ ACP）,alkaline phosphatase（ AKP） activities in different tissues and the contents of serum cytokines of Pseudosciaena crocea were studied. The experiment was divided into three experiment groups（ D1-100 mg/kg,D_2-150 mg/kg,D3-300 mg/kg） and one control group. The samples were respectively collected on the 30 thdays and the 45 thdays. The results indicated that,compared with the control group,when the dietary curcumin supplement level was 100 mg/kg,the ACP activities of liver and proximal intestine plus with the AKP activities of gill,stomach,muscle and liver increased significantly（ P 0.05,the same as below）.The dietary curcumin supplement level of 150 mg/kg affected the activities of ACP in muscle,liver and proximal intestine as well as the activities of AKP in gill,stomach,muscle,liver and proximal intestine. When dietary curcumin supplement level was 300 mg/kg,the ACP and AKP activities of distal intestine plus with the AKP activity of mid intestine increased insignificant（ P 0.05,the same as below）. At the same time,the ACP and AKP activities in other tissues increased significantly. In addition,compared with the control group,only the contents of TNF-α increased significantly when the dietary curcumin supplement level was 100 mg/kg. The contents of GM-CSF,IL-2 and TNF-α all increased significantly when the dietary curcumin supplement level was 150 or 300 mg/kg.Besides,in term of the contents of IFN-γ,there were no significant differences among the three groups,but they decreased in the two periods. On the whole,the dietary curcumin supplement level of 300 mg/kg had a better effect on phosphatase activities in different tissues and the contents of serum cytokines in Pseudosciaena crocea.