萝藦花总黄酮的含量测定及抗氧化研究

On the Determination and Antioxidant Activity of the Total Flavonoids in Japanese Metaplexis Flowers

利用分光光度法测定了野生萝藦花总黄酮含量,研究了萝藦花总黄酮的抗氧化能力。结果表明：采用Al（NO3）3-NaNO2-NaOH显色体系测定萝藦花总黄酮含量,最佳测定时间为显色后70-80min,测定方法精密度RSD=0.99%（n=5）,回收率为107.43%（RSD=2.40%）,利用该法测得萝藦花样品总黄酮含量为2.41%（RSD=3.02%,n=5）;制得的萝藦花黄酮对·OH、DPPH·、ABTS·＋清除作用的IC50分别为25.06μg/mL、9.94μg/mL、1.33μg/mL,最大清除率分别为57.87%、85.79%、99.52%。

The content of total flavonoids in wild Japanese metaplexis flowers was determined using Al（NO3）3-NaNO2-NaOH chromogenic system and spectrophotometry,and its oxidation resistance is studied.The results showed that the optimal time to scan the absorption spectrum was 70 min≤t≤80min,the relative standard deviation（RSD）of precision test was 0.99%（n=5）,the mean recovery rate was 107.43%（RSD=2.40%）,and total flavonoids yielded in Japanese metaplexis flowers sample was2.41%（RSD=3.02%,n=5）;in the scavenging effect of the flavonoids from Japanese metaplexis flowers on ·OH,·DPPH,and ABTS·＋,the IC50 were 25.06μg/mL,9.94μg/mL,and 1.33μg/mL,and the maximum scavenging rates were 57.87%,85.79%,and 99.52%respectively.