聚耐尔蓝/氧化石墨烯光电极对NADH的光致电化学响应

Photoelectrochemical response of poly-nile blue/graphene oxide photoelectrode to NADH

在氧化石墨烯修饰的ITO电极上,通过电聚合光敏材料耐尔蓝,构建了具有电子受体功能的光电极.该光电极能与电子供体NADH（烟酰胺腺嘌呤二核苷酸）产生光致电化学反应,通过测量反应产生的光电流实现了对NADH的检测.探讨了光致电化学响应机理,研究了氧化石墨烯固定量和耐尔蓝初始浓度对光电极性能的影响.在优化了电解质溶液pH和偏压的测试条件下,该光电极对NADH的响应范围为0.01~80.0μmol/L,检出限为7.0nmol/L,相对标准偏差小于2.73%.该光电极的制备和对NADH的检测不需要除氧,有经济、简便等优点.

In the paper,the nile blue （Nb）, a photosensitive compound was electropolymerized on the surface of ITO electrode modified by grapheme oxide （GOx）, to prepare a p-Nb/GOx photoelec- trode. The photoelectrode as photosensitive interface and electron acceptor can occur to photoelec- tronchemical （PEC） reaction with NADH （nicotinamide adenine dinucleotide） as electron donor. The quantitative determination of NADH was achieved by monitoring photocurrent based on PEC re- sponse of photoelectrode to NADH. In the paper,the reaction mechanism was investigated between the photoelectrode and NADH. The influences of the solution pH and bias voltage on the detection of NADH were studied. Under the optimized conditions, the photocurrent is proportional to the log- arithm of NADH concentration in the range of 0.01-80.0 μmol/L,and the detection limit was esti- mated to be 7.0 nmol/L （S/N=3）. The regression equation is △i（nA）=404. 7＋81. 76 lgc （μmol/L） with correlation coefficient of 0. 993. The relative standard deviation （RSD） is less than 2.73% for five parallel measurements of same sample.