摘要
目的探讨3-D多孔结构的小肠黏膜下组织(small intestine submucosa,SIS)在新西兰大白兔膀胱再生中的应用。方法用物理及化学方法制备具有3-D多孔结构的SIS。将24只雄性新西兰大白兔随机分为3组(n=8),分别行膀胱半切术建立膀胱缺损模型,使用3种不同处理的SIS进行膀胱重建。A组:未经过氧乙酸(peroxyacetic acid,PAA)处理的SIS组;B组:PAA处理的SIS组;C组:PAA处理后100%胎牛血清浸润的SIS组。3组在术前及术后4周测定膀胱容量,并于术后4周取材,行大体观察及组织学检测,评估膀胱再生情况。结果应用SIS行兔膀胱重建术后4周,3组再生膀胱组织与周围组织粘连依次降低,结石率分别为33.3%、28.6%、14.31%。与术前自身比较,术后4周3组膀胱最大容量均缩小且依次增加,组间比较差异有统计学意义(P<0.05)。术后组织学检测示3组膀胱上皮细胞均有再生,与A组比较,B、C组的上皮再生更好,边缘区和中心区无明显差异。3组膀胱平滑肌的再生均不理想,但边缘区平滑肌再生较中心区多;在边缘区和中心区,组间比较差异有统计学意义(P<0.05)。3组在边缘区和中心区的再生血管面积依次增加,3组间比较差异有统计学意义(P<0.05)。结论经PPA及胎牛血清处理后3-D多孔结构的SIS,具有良好的促进膀胱组织再生能力,是一种比较理想的修复支架材料。
Objective To investigate the application of 3-D microporous small intestinal submucosa (SIS) in bladder regeneration of a rabbit model. Methods 3-D microporous SIS was made with physical and chemical method. A total of 24 male New Zealand white rabbits were inflicted with partial cystoplasty and then randomly divided into 3 groups ( n = 8 for each group) and respectively treated with 3 types of SIS for bladder reconstruction. They were group A repaired with treated SIS without peroxyacetic acid (PAA), group B with PAA treated SIS, and group C with PAA treated SIS and then with 100% fetal bovine serum infiltration. The bladder capacity was measured preoperatively and in 4 weeks postoperatively. Then the rabbits were sacrificed for gross observation and histological examination of bladder in order to evaluate the bladder regeneration. Results In 4 weeks after partial cystoplasty, the tissue adhesion with the surrounding was reduced in turn in groups A, B and C, and the stone formation rate was 33.3% , 28.6% and 14.31% respectively. The bladder capacity was decreased first and then increased in turn in the 3 groups, and significant difference was seen among the 3 groups (P 〈 0.05 ). Histological observation revealed that urothelial cells were well regenerated in the 3 groups, with those of the groups B and C better than those of group A, and no difference was seen between the marginal zone and the central zone. However, smooth muscle regeneration was not ideal in the 3 groups, but those in the marginal zone were better than the cells in the central zone with significant difference in the groups (P 〈 0.05 ). The regeneration area of blood vessels were increased in groups A, B and C in turn, and there was statistically significance in the groups ( P 〈 0.05 ). Conclusion 3-D microporous SIS treated with PAA and fetal bovine serum has ability of promoting the bladder tissue regeneration, and can be regarded as an ideal alternative to repair materials.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2016年第13期1487-1493,共7页
Journal of Third Military Medical University
基金
国家自然科学基金面上项目(81371704)
重庆高校创新团队建设计划资助项目(81370701)~~
关键词
组织工程
小肠黏膜下组织
膀胱再生
血管化
tissue engineering
small intestinal submucosa
bladder regeneration
revascularization