针刺关元穴对寒凝证类痛经大鼠扭体反应和血管舒缩物质的影响

Effect of Acupuncture at Guanyuan(CV4) on Writhing Response and Vasomotor Substances in Rats with Dysmenorrhea Due to Coagulated Cold Syndrome

目的观察针刺关元穴对寒凝证类痛经大鼠镇痛效应及血管舒缩物质的影响。方法本实验用注射苯甲酸雌二醇和缩宫素再结合全身冷冻的方法制备寒凝证类痛经大鼠模型,观测大鼠扭体反应(扭体潜伏期、扭体次数、扭体评分),并用酶联免疫吸附法测定血浆TXB_2和6-keto-PGF1a含量。结果与盐水组比较,模型组扭体潜伏期明显缩短,扭体次数明显增多,扭体评分明显升高(P<0.01);与寒凝证类痛经模型组比较,针刺组扭体潜伏期均明显延长,扭体次数均明显减少,扭体评分均明显降低(P<0.05,P<0.01)。与盐水组比较,模型组血浆6-keto-PGF_(1a)含量明显降低(P<0.05);血浆TXB_2含量有上升趋势(P>0.05)。与模型组比较,针刺(关元)组血浆6-keto-PGF_(1a)含量有升高趋势(P>0.05);血浆TXB_2含量有降低趋势(P>0.05)。结论寒凝证类痛经大鼠血液中血管舒缩物质出现明显紊乱。针刺关元穴可在改善痛经大鼠扭体反应缓解痛经程度的同时,良性调节TXB_2及6-keto-PGF_(1a)。血管舒缩物质可能是针刺防治痛经的重要物质之一。

Objective To observe the analgesic effect of acupuncture at Guanyuan（CV4） and its effect on vasomotor substances in rats with dysmenorrhea due to coagulated cold syndrome. Method The coagulated-cold dysmenorrhea rat model was developed by Estrodiol benzoate and Oxytocin injectin plus physical freezing. The writhing response（writhing latency, writhing frequency, and writhing score） was observed, and the contents of TXB_2 and 6-keto-PGF_（1a） were detected by using enzyme-linked immunosorbent assay（ELISA）. Result Compared with the saline water group, the writhing latency was significantly shortened, the writhing frequency was significantly increased, and the writhing score was more significantly increased in the model group（P〈0.01）; compared with the model group, the writhing latency was significantly prolonged, the writhing frequency was decreased, and the writhing score was significantly lower in the acupuncture group（P〈0.05, P〈0.01）. Compared with saline water group, the content of plasma 6-keto-PGF_（1a） was significantly lower（P〈0.05） and the content of plasma TXB_2 showed an increasing tendency（P〈0.05） in the model group. Compared with the model group, the content of plasma 6-keto-PGF_（1a） showed an increasing tendency（P〈0.05） and the content of plasma TXB_2 showed a decreasing tendency（P〈0.05） in the acupuncture group. Conclusion The vasomotor substances are obviously disordered in the blood of cold-coagulated dysmenorrhea rat models. Acupuncture at Guanyuan can improve the writhing response and release pain, and meanwhile positively regulate the vasomotor substances such as TXB_2 and 6-keto-PGF_（1a）. The vasomotor substances are plausibly one of the major substances in the action of acupuncture in preventing and treating dysmenorrhea.