松果菊苷通过调控GFRα1/AKT信号通路抑制MPP^+诱导的多巴胺能细胞SH-SY5Y凋亡

Echinacoside inhibits apoptosis of SH-SY5Y cell through GFRα1/AKT pathway

目的研究肉苁蓉提取物松果菊苷(echinacoside)对1-甲基-4-苯基吡啶(MPP+)诱导帕金森病模型SH-SY5Y细胞的保护机制。方法 SH-SY5Y细胞分别接受磷酸缓冲溶液(PBS)、MPP+和/或松果菊苷处理后,分析细胞生长情况,采用Western blot结合免疫荧光法分析各处理组细胞内胶质细胞源性神经营养因子家族受体α1(GFRα1)及其下游抗凋亡AKT(蛋白激酶B)磷酸化及半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)水平变化,并观察AKT抑制剂LY249002对松果菊苷对SH-SY5Y的保护功能的影响。结果松果菊苷可以显著缓解MPP+诱导的SH-SY5Y细胞的凋亡[MPP+vs MPP+/松果菊苷,(25.12±4.33)%vs(5.13±1.51)%,P<0.01]。松果菊苷可以抑制MPP+下调SHSY5Y细胞内GFRα1表达和AKT磷酸化。进一步的研究表明特异性阻断AKT信号通路可以取消松果菊苷促进SHSY5Y在MPP+诱导损伤下生存的功能,但不影响松果菊苷提高GFRα1的表达。对SH-SY5Y细胞内Caspase-3活性分析的结果显示,松果菊苷可以抑制MPP+诱导Caspase-3的活化[MPP+vs MPP+/松果菊苷,(7.22±1.51)vs(1.81±0.42),P<0.01],但是这一作用可以被AKT抑制剂阻断。结论松果菊苷通过上调GFRα1/AKT通路抑制MPP+诱导的SH-SY5Y细胞凋亡,发挥保护神经细胞存活的功能。

AIM To study the protective effects of echinacoside,extracted from Cistanches Herba,on 1-methyl-4-phenylpyridiniumion（MPP^＋）-induced SH-SY5 Y cells of Parkinson＇s disease model.METHODS After SH-SY5 Y cells were treated with phosphate buffer saline（PBS）,40 μg/m L echinacoside,1 mmol/L MPP^＋,and/or both of echinacoside and MPP^＋,cell proliferation was detected,and GFRα1,phosphorylated AKT molecule（protein kinase B） as well as Caspase-3 protein level were analyzed by Western blot and immunofluorescence.Echinacoside＇s protective effects were also evaluated when AKT phosphorylation was blocked by an AKT specific inhibitor,LY49002.RESULTS Echinacoside significantly decreased cell apoptosis induced by MPP^＋in SHSY5 Y cells [MPP^＋vs MPP^＋/echinacoside,（25.12 ± 4.33） % vs（5.13 ± 1.51） %,P〈0.01].Data indicated that echinacoside could up-regulate the expression of GFRα1,a critical pro-survival receptor,and improve AKT phosphorylation after SH-SY5 Y cells were exposed to MPP^＋.Further study suggested that LY49002 could abrogate echinacoside＇s function of improving cell survival but not affecting its effect of increasing GFRα1.Echinacoside also could inhibit Caspase-3 activation induced by MPP^＋in SH-SY5 Y [MPP^＋vs MPP^＋/ECH,（7.22 ± 1.51） vs（1.81 ± 0.42）,P〈0.01].But its function depended on AKT activation.CONCLUSION In summary,our data strongly support that echinacoside protects cell against MPP^＋-induced apoptosis through GFRα1/AKT signal pathway in SH-SY5 Y cells.