非离子表面活性剂对鞘氨醇单胞菌MH13转化吩嗪的影响

Effect of Nonionic Surfactants on the Biotransformation of Phenazine by Sphingobium sp. MH13

1,2-二羟基-1,2-二氢吩嗪是一种手性的二醇,可以被用来作为合成吩嗪类衍生物的前体。鞘氨醇单胞菌MH13能将吩嗪转化为1,2-二羟基-1,2-二氢吩嗪。利用添加非离子表面活性剂的方法对鞘氨醇单胞菌MH13转化吩嗪进行了研究。首先考察了不同种类的非离子表面活性剂对菌株生长的影响。结果表明2%的Triton X-100和Triton X-114抑制鞘氨醇单胞菌MH13的生长,而2%的聚醚L64对鞘氨醇单胞菌MH13毒性较小。然后选用2%的聚醚L64作为添加剂,在培养24h后中间产物1,2-二羟基-1,2-二氢吩嗪产量达到最大,浓度达到了(98.9±7.1)mg/L,是不添加非离子表面活性剂的1.32倍。

1,2-Dihydroxy-1,2-dihydrophenazine（DHDP）is a kind of chiral diol which can be used as a precursor for synthesizing phenazine derivatives.Sphingobiumsp.MH13 can transform phenazine to 1,2-dihydroxy-1,2-dihydrophenazine.The effect of nonionic surfactants on the biotransformation of phenazine by sphingobiumsp.MH13 was investigated.Firstly,the effects of different kinds of nonionic surfactant on cell growth were studied.The result showed that the addition of 2% Triton X-100 and Triton X-114 inhibited the growth of sphingobiumsp.MH13,while 2% polyether L64 showed little toxicity to sphingobium sp.MH13.Then polyether L64 was selected as the additive for the biotransformation of phenazine.After adding 2% concentration of polyether L64 for 24h,the production of DHDP reached the maximum as（98.9±7.1）mg/L,which was 1.32 times more than that of the control.