高效液相色谱法同时测定葡萄籽提取物中儿茶素、原花青素B1和原花青素B2的含量

Simultaneous determination of catechin, procyanidins B1 and procyanidins B2 in grape seed extract by HPLC

目的建立高效液相色谱法同时测定葡萄籽提取物中儿茶素、原花青素B1和原花青素B1的含量。方法色谱柱为ZorbaxSBC。流动相为乙腈-0．5％冰醋酸溶液（梯度洗脱），检测波长为278nm，柱温为35％，流速为1．0ml／min。结果儿茶素、原花青素B1和原花青素B2的质量浓度分别在0．99～66．0（r=0．9999）、0．774．51．6（r=0．9998）、1．44～96．0（r=0．9999）mg／L范围内呈良好的线性关系，精密度试验及重复性、稳定性试验的相对标准偏差（RSD）均小于3％，平均加样回收率分别为99．48％、95．95％、99．14％，RSD分别为0．90％、0．91％、2．72％（n=6）。结论本方法经验证，可用于葡萄籽提取物中儿茶素、原花青素B1和原花青素B2含量的测定。

Objective To establish an HPLC methodology for simultaneous determination of catechin, procyanidins B1 and procyanidins B2 in grape seed extract. Methods The determination was performed on a Zorbax SB Cl8 column with mobile phase consisting of eetonitrile -0.5% acetic acid （gradient elution）. The detection wavelength was set at 278 nm, and column temperature was 35°C. The flow rate was 1.0 ml/min. Results The linear range was 0.99 - 66.0 mg/L for catecbin （ r = 0. 999 9 ）, 0. 774 ～ 51.6 mg/L for procyanidins B1 （ r = O. 999 8 ） and 1.44 - 96.0 mg/L for pro- cyanidins Bz （r = 0. 999 9 ）. The relative standard derivation （RSD） was 0.90% for precision test, 0.91% for repeat- ability test and 2.72% for stability test. The average recovery was 99.48% , 95.94% and 99.14%, respectively. Con- dusion The developed methodology is verified to determine the content of cateehin, procyanidins Bt and procyanidins B2 in grape seed extract.