CD44、CD24和PCNA与乳腺癌腋窝淋巴结转移关系研究

Relationship among CD44, CD24, PCNA and axillary lymph node metastasis in breast cancer

目的探讨CD44、CD24和增殖细胞核抗原（PCNA）与乳腺癌淋巴结转移之间的关系。方法乳腺癌切除组织标本120例，分为①前哨淋巴结阴性病例组、②前哨淋巴结阳性腋窝淋巴结未转移病例组、⑧前哨淋巴结阳性腋窝淋巴结转移病例组。分别对乳腺癌病理石蜡存档组织标本进行CD44、CD24和PCNA免疫组化标记，分析CD44、CD24及PCNA与淋巴结转移之间的关系。结果腋窝淋巴结转移组中：PCNA及CD44＋／CD24 -/loW同时表达阳性细胞病例数与其他两组比较明显增高，差异具有统计学意义（P〈0．05）；CD44、PCNA、CD24阳性表达例数均显著高于无腋窝淋巴结转移的其他两组，差异具有统计学意义（P均〈0．05）。结论乳腺癌组织中CD44、CD24、PCNA阳性表型细胞比例决定腋窝淋巴结转移程度。

Objective To explore the relationship among CD44, CD24, proliferating cell nuclear antigen （PCNA） and lymph node metastasis of breast cancer. Methods A total of 120 breast cancer samples were divided into the follow- ing groups： a sentinel lymph node negative group, a sentinel lymph node group where no metastasis was found in the posi- tive axillary lymph nodes, and a sentinel lymph node where metastasis was found in the positive axillary lymph nodes. Then, CD44, CD24 and PCNA within the samples were immunohistochemically stained, in order to analyze the relation- ship among CD44, CD24, PCNA and lymph node metastasis. Results Patients with metastasis in the axillary lymph node produced a remarkably higher number of cells with positive staining of PCNA and CIM4 ＋/CD24-/loW than those in other two groups （P 〈0.05 ） and their number of cells with positive CD44, CD24 and PCNA staining was significantly higher than those in other two groups （P 〈 0.05 ）. Conclusion The proportion of cells with CD44, CD24 and PCNA positive phenotypes in breast cancer determines the degree of axillary lymph node metastasis.