火炬松松针多糖提取工艺及其抗氧化性研究

Extraction Process and Antioxidant Activity of Pinus taeda L.Polysaccharides

以多糖得率为指标,从火炬松松针中浸提多糖,并测定其体外抗氧化活性。在单因素试验基础上采用响应面法优化超声波辅助热水浸提火炬松松针多糖,最佳的工艺参数为:30 g松针粉末在超声波作用时间25 min,热水浸提1 h,液料比25∶1(m L∶g),热水浸提温度91℃。在此条件下,火炬松松针多糖得率达1.867%,提取率达91.39%。通过测定松针多糖对苯基苦基肼自由基(DPPH·)、羟基自由基(·OH)和2,2-联氮-二-(3-乙基-苯并噻唑-6-磺酸)自由基(ABTS+)的清除能力评价其体外抗氧化能力。结果显示:火炬松松针多糖对自由基DPPH·、·OH和ABTS+都有较强清除能力,且都呈较好的量效关系,火炬松松针多糖具有较强的体外抗氧化能力。

With the polysaccharides yield as target,the polysaccharides from pine needles of Pinus taeda L.was extracted and its antioxidant activity in vitro was determined.The ultrasonic-assisted extraction technology of polysaccharides from pine needles of P.taeda was confirmed by using Box-Behnken response surface methodology.The results showed that the optimal extraction process conditions were ultrasonic time 25 min,water extraction time 1 h,liquid-material ratio of 25 ∶ 1（m L ∶ g） and extraction temperature 91 ℃.Under optimal conditions,the yield of polysaccharides was up to 1.867%.The antioxidant activity in vitro was evaluated by measuring the DPPH·,·OH and ABTS＋free radical removal ability.As a result,the pine needle polysaccharides had a relatively strong capacity to scavenge DPPH,OH and ABTS＋free radical.They had a better concentrationresponse relationship.It showed that pine needle polysaccharides had an obvious antioxidant activity.