pS6和pS6K蛋白在结直肠癌中的表达及其对预后影响

Expression of pS6 and pS6K Protein in Colorectal Carcinoma and Its Prognostic

目的探讨p S6和p S6K蛋白在结直肠癌组织中的表达变化及其临床意义,并分析其对预后的影响。方法采用免疫组织化学方法检测120例结直肠癌组织和癌旁正常组织中p S6和p S6K蛋白的表达情况,分析p S6和p S6K蛋白的表达变化与结直肠癌患者临床病理指标及预后的关系。结果 p S6和p S6K蛋白在结直肠癌组织中的阳性表达率分别为62%和65%,而在癌旁切缘组织中的阳性表达率分别为5%和3%。阳性表达的p S6和p S6K与肿瘤的分化程度(分化好与差)之间有统计学意义(P=0.002和P=0.001),p S6K的高表达与结直肠癌的淋巴结转移显著正相关(P=0.016),而与患者的性别、年龄、肿瘤的病理等级无关(P>0.05)。生存分析的结果显示,p S6和p S6K均是阳性的结直肠癌患者预后显著差于其它组患者,差异有统计学意义(P=0.007)。结论 p S6和p S6K蛋白在结直肠癌中表达增加,可能作为结直肠癌预后判断的指标。

Objective To investigate the expression change and clinical significance of pS6 and pS6K protein in colorec- tal cancer tissues, and to analyze its influence on prognosis. Methods Immunohistochemical method was used to detect PS6 and pS6K protein expression in coloreetal cancer tissues and adjacent normal tissues of 120 cases, and the relationship between expres- sion changes of pS6 and pS6K protein and clinicopathological parameters as well as prognosis of colorectal cancer patients was ana- lyzed. Results Positive expression rates of pS6 and pS6K protein in colorectal cancer tissue were 62% and 65v respectively, while in the adjacent normal tissue the positive expression rates were 5% and 3% respectively. Positive expression of PS6 and pS6K had statistically significant relationship with degree of tumor differentiation（ well or poor differentiated） （P = 0. 002 and P = 0. 001 ）. High expression of pS6K had significantly positive correlation to lymph nodes metastasis of colorectal cancer （P = 0. 016）, but no correlation to sex, age and tumor pathological grade of patients （P 〉 0. 05 ）. Results Of survival analysis showedthat colorectal cancer patients with double positive results of pS6 and pS6K had significantly worse prognosis than patients of other groups（P = 0. 007）. Conclusion Increased expression of pS6 and pS6K protein in colorectal cancer may serve as an indicator of the prognosis of colorectal cancer.