电针对神经病理性痛大鼠脊髓及背根神经节中白细胞介素-33和ST2表达的影响

Effect of electroacupuncture on the expressions of IL-33 and ST2 in spinal cord and dorsal root ganglion in rats with neuropathic pain

目的探讨电针对神经病理性痛大鼠脊髓及背根神经节中白细胞介素（IL）-33和ST_2表达的影响。方法成年雌性SD大鼠40只,体重190~240g,随机均分为假手术组（Sham组）、模型组（MC组）、同侧电针组（SE组）和对侧电针组（VE组）。建立坐骨神经压迫性损伤模型,SE组和VE组大鼠分别对右侧肢体和左侧肢体的阳陵泉穴和足三里穴进行电针治疗,分别于治疗前（T0）、治疗7d（T_1）、治疗后3d（T_2）和治疗后7d（T_3）测定四组大鼠机械缩足阈值（MWT）和热缩足潜伏期（TWL）,于T_3时采用荧光定量PCR检测大鼠脊髓及背根神经节中IL-33和ST_2表达。结果与T0时比较,T_1~T_3时MC组、SE组和VE组大鼠MWT明显降低,TWL明显缩短（P〈0.05）;T_1时MC组,T_1~T_3时SE组和VE组大鼠MWT明显低于Sham组,T_1~T_3时MC组、SE组和VE组大鼠TWL明显短于Sham组（P〈0.05）;T_2、T_3时SE组和VE组大鼠MWT明显高于,TWL明显长于MC组（P〈0.05）。MC组、SE组和VE组大鼠脊髓及背根神经节中IL-33 mRNA和ST_2mRNA相对表达量明显高于Sham组（P〈0.05）;SE组和VE组大鼠脊髓及背根神经节中IL-33mRNA和ST_2mRNA相对表达量明显低于MC组（P〈0.05）。结论电针治疗可明显减轻神经病理性疼痛大鼠痛,可能通过抑制脊髓及背根神经节中IL-33和ST_2表达,阻断IL-33/ST_2介导的炎性反应而发挥作用。

Objective To investigate the effect of electroacupuncture on the expressions of in-terleukin（IL）-33 and ST2 in spinal cord and dorsal root ganglion in rats with neuropathic pain. Methods Forty adult female SD rats were randomly divided into sham operation group （group Sham）,model group （group MC）,ipsilateral electroacupuncture group （group SE）and contralateral electroacupuncture group （group VE）.Each group had 10.The sciatic nerve injury models were es-tablished.The rats in group SE and group VE were respectively accepted electrical acupuncture treat-ment in Yanglingquan and Zusanli in the right and left limbs.Respectively,before treatment （T0 ）, treated for 7 d （T1 ）,3 d （T2 ）and 7 d （T3 ）after treatment,the MWT and TWL of rats in the four groups were measured.At T3 ,the expressions of IL-33 in spinal cord and dorsal root ganglion in rats were detected by using quantitative PCR.Results Compared with T0 ,MWT and TWL in group MC, group SE and group VE at T1-T3 were decreased significantly （P 〈 0.05）.Compared with sham group,MWT in group SE and group VE,TWL in group MC,group SE and group VE at T1-T3 were decreased significantly （P 〈0.05）.Compared with group MC,MWT and TWL in group SE and group VE at T2 ,T3 were increased significantly （P 〈0.05）.Compared with sham group,the relative expression levels of IL-33 mRNA and ST2 mRNA in spinal cord and dorsal root ganglion in the group MC,group SE and group VE were elevated,the differences were statistically significant （P 〈0.05）. Compared with group MC,the relative expression levels of IL-33 mRNA and ST2 mRNA in spinal cord and dorsal root ganglion group SE and group VE were decreased,the differences were statistical-ly significant （P 〈0.05）.Conclusion The electroacupuncture treatment could significantly reduce the pain symptoms of neuropathic pain in rats,possibly via inhibiting the expressions of IL-33 and ST2 in＆nbsp;spinal cord and dorsal root ganglion and blocking the IL-33/ST2 mediated inflammatory to play role.