摘要
目的:夏枯草为夏桑菊颗粒的君药,研究检测夏枯草主要药效成分迷迭香酸来建立夏桑菊的质控指标。方法:采用HPLC方法对夏桑菊中迷迭香酸含量进行测定,检测条件为色谱柱:Zorbax Eclipse XDB-C_(18);流动相:乙腈-1%醋酸(19∶81)洗脱;流速:1.0ml/min;柱温:40℃;检测波长:329nm;进样量:10μl。结果:检测得到迷迭香酸含量平均值为0.33mg/g,该方法的平均回收率为101.01%,线性方程为Y=2376737X-3261。结论:为提高夏桑菊颗粒质量标准,对处方中夏枯草以迷迭香酸计的含量进行研究,为更好的监控和评价夏桑菊颗粒的质量提供实验依据。
Objective Prunella valgaris is the monarch drug of Xiasangju granules,in this study to establish a quality control for Xiasangju granules by detecting rosmarinic acid in Prunella.Methods Determine the rosemary acid in Xiasangju granulesby HPLC, the detection conditions,Column Zorbax Eclipse XDB -C18;mobile phase：acetonitrile -1% acetic acid （19∶81）to give;flow rate：1.0ml/min;column temperature：40℃;detection wavelength：329nm;injection volume：10μl.Results The average content of rose-mary acid was 0.33mg/g,the average recovery of the method was 101.01%,the linear equation was Y=2376737X -3261.Conclu-sion This study can improve Xiasangju pranules quality standards,prescription Prunella meter with rosemary acid content were studied to provide a scientific basis for better quality monitoring and evaluation Xiasangju granules.
出处
《中国民族民间医药》
2016年第12期44-46,共3页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
科技部“重大新药创制”(2013ZX09201019)
公益性行业科研专项(201507002)
湖南省十二五重点学科药学学科资助
