芹菜素诱导膀胱癌5637细胞凋亡研究

Effects of apigenin on apoptosis of human bladder cancer 5637 cells

目的：研究芹菜素对人膀胱癌5637细胞增殖及凋亡的影响。方法：采用20-80μmol/L芹菜素处理5637细胞,MTT法检测膀胱癌5637细胞增殖抑制率;细胞软琼脂克隆形成实验检测细胞成瘤能力的抑制效率;Hoechst 33258细胞核染色法观察细胞凋亡的形态学改变。结果：芹菜素呈浓度依赖的抑制5637细胞的增殖;80μmol/L芹菜素处理组细胞出现核固缩、染色质凝集和核碎片化等典型的细胞凋亡特征;5637细胞的克隆形成能力随着芹菜素浓度增加而显著降低。结论：芹菜素能够抑制人膀胱癌5637细胞的增殖,抑制5637的克隆形成,并诱导细胞凋亡。

Objective To study the effects of apigenin on the apoptosis of human bladder cancer cell line 5637.Methods 5637 cells were cultured with different concentrations of apigenin,the MTT assay was used to evaluate the cell inhibition rates.Apoptosis of 5637 cells was observed under a fluorescence microscope using Hoechst 33258 staining,5637 tumorigenicity was messaured by soft colony formation assay.Results Apigenin causes concentration -dependent inhibition of the proliferation of bladder cancer 5637 cells. 5637 cells treated with apigenin showed significant morphological changes of apoptosis,and the cell tumorigenicity was inhibited as apigenin concentration increased.Conclusion Apigenin can inhibit the proliferation and induce apoptosis of 5637 cells.