枸杞子贮藏过程中水分等生理活性变化与“走油”的相关性

Changes of Moisture and Relativety of ‘Oil-spilling' During Storage of Lycii Fructus

目的:研究枸杞子贮藏过程中水分含量、活性氧自由基清除酶超氧化物歧化酶(SOD),过氧化物酶(POD)活性和脂质氧化终产物丙二醛(MDA)含量变化与"走油"的相关性,为阐明枸杞子"走油"机制,建立其科学贮藏方法提供依据。方法:将枸杞子药材分别在40℃湿度75%和4℃湿度60%2种条件下贮藏,于0,20,40,60 d取样,观测其外观性状,测定其多糖、水分、MDA含量和SOD,POD活性。多糖和水分的含量测定采用2010年版《中国药典》枸杞子项下方法,MDA含量测定采用TBA法,SOD活性测定采用氯化硝基四氮唑蓝光还原法,POD活性测定采用愈创木酚法。结果:在40℃湿度75%条件下贮藏的枸杞子在第20天即开始"走油",随着贮藏时间的延长,"走油"程度逐渐加深,水分不断增加,SOD,POD活性相应降低,MDA的含量不断升高。而在4℃湿度60%条件下贮藏的枸杞子外观性状,水分,多糖,MDA含量及SOD,POD活性均无明显变化。结论:"走油"是外在贮藏条件不当,致使内在水分含量增加,活性氧自由基增多,细胞结构和功能受损,细胞膜脂过氧化,失去选择透性的结果。

Objective： To study the changes of the water content,superoxide dismutase（ SOD）,peroxidase（ POD） and lipid oxidation end-products of malondialdehyde（ MDA） during the storage of Lycii Fructus,to study the mechanism of ‘Oil-spilling ＇,and establish its scientific storage methods. Method： Two storge methods,including temperature at 40 ℃,humidity of 75%, temperature at 4 ℃,humidity of 60%,respectively. Observed the appearance of Lycii Fructus at 0,20,40,60 days. While measured polysaccharide and water by Chinese Pharmacopoeia 2010 edition under the medlar method. MDA was tested by TBA method,SOD activity was measured by nitroblue tetrazolium reduction blue,POD was measured by nordihydroguaiaretic phenol method. Result： ‘Oil-spilling＇was started from 20 day at the storage of 40 ℃,humidity of 75%,inhibited timedependent,while the moisture and MDA content were increased,SOD and POD activities were decreased. At the same time,there＇s no significantly changes under the storage of 4 ℃,humidity of 60%. Conclusion： Imoroer storage and the water content and reactive oxide species of wolfberry increased. Result in the cell structure and function damaged,cell membrane lipid peroxidated,or permeability changed.