Foxp3基因多态性与广东地区产后抑郁相关性的初步探讨

Explore the association between the polymorphism of Foxp3 gene and postpartum depression in Guangdong Province

目的探讨中国广东地区女性叉头状螺旋转录因子3(Foxp3)-6054(deletion/ATT,rs5902434)、Foxp3-3279(A/C,rs376158)、Foxp3-924(A/G,rs2232365)与产后抑郁症(PPD)发病的相关性。方法用聚合酶链反应-序列特异性引物(PCR-SSP)分析法对69例PPD确诊患者(PPD组)和140例健康产后女性(对照组)的外周血提取DNA样本,进行Foxp3-6054、Foxp3-3279、Foxp3-924点突变分析,比较两群体该基因多态性的差异。结果 Foxp3基因Foxp3-6054位点在PPD组AA基因型频率和对照组间差异有统计学意义(P<0.05),Foxp3-6054位点等位基因频率、Foxp3-3279、Foxp3-924位点单核苷酸多态性基因型频率和等位基因频率在PPD组和对照组间差异无统计学意义(P>0.05)。结论中国广东地区Foxp3-6054与PPD的发病具有一定的相关性,中国广东地区Foxp3-3279、Foxp3-924与PPD的发病未发现相关性。

Objective To investigate the association of Fork shaped spiral gene transcription factor 3（Foxp3）-6054（deletion/ATT,rs5902434）,Foxp3-3279（A/C,rs376158）,Foxp3-924（A/G,rs2232365）with the morbidity of postpartum depression among the populations in Guangdong province.Methods The peripheral blood DNA samples were identified by means of polymerase chain reaction sequence specific primers（PCR-SSP）analysis among 69 cases which were confirmed to be postpartum depression and 140 cases of healthy control of postpartum women,and then we analyzed the point mutation of Foxp3-6054,Foxp3-3279,Foxp3-924 as well as compare the differences between the two groups in gene polymorphisms.Results The SNP gene frequency and genotype frequency of Foxp3-6054 was statistical significant among the group of postpartum depression and the control group（P〉0.05）,and the SNP gene frequency and genotype frequency of Foxp3-3279,Foxp3-924 was not statistical significant among the group of postpartum depression and the control group（P0.05）.Conclusion From the current investigation cases,the Foxp3-6054 could be proved to associated with the morbidity of postpartum depression among the populations in Guangdong province,and the Foxp3 gene Foxp3-3279,Foxp3-924 could not be proved to associated with the morbidity of postpartum depression among the population in Guangdong province.